In Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings.

This volume collects new information on the genomics of saprophytic soil Pseudomonas, as well as functions related to genomic islands. It explores life styles in different settings and sheds further insights on the wide metabolic potential of this microbe for the removal of pollutants and production of added-value products. This volume also explores how Pseudomonas responds and reacts to environmental signals, including detection of cell density.

Pseudomonas volume 7 collects some of the most relevant and emerging issues in the biology of these microorganisms, and a number of other important issues that were not collected in the previous volumes. The first six volumes of the Pseudomonas series covered the biology of pseudomonads in a wide range of contexts, including the niches they inhabit, the taxonomic relations among its members of this group, the molecular biology of gene expression in different niches and under different environmental conditions, the analysis of virulence in plants, animal and human pathogens, as well as the determinants that make some of these strains of interesting for biotechnological applications. This seventh volume covers the following topics: The history of the biology of Pseudomonas The use of Pseudomonas as biological agents New trends in the molecular biology of these microorganisms Pseudomonas and the immune system of insects and animals This book will be of use to researchers working on these bacteria, particularly those studying medical aspects of Pseudomonas, and their use as a means to control pathogens or to stimulate plant growth. This volume is also interesting for those studying the physiology, genetics, molecular biology of Pseudomonas and those using novel-omics approaches to understand bacteria of the genus Pseudomonas.

TwentyyearshavegonebysinceJackSokatch?rstpublishedhisoutsta- ingTheBiologyofPseudomonasbackin1986.Thiswasfollowedbytwobooks published by the ASM that contained the presentations of the Pseudomonas meetings held in Chicago in 1989 and Trieste in 1991. The earlier volume of these two was edited by Simon Silver, Al Chakrabarty, Barbara Iglewski, and Sam Kaplan, and the later one by Enrica Galli, Simon Silver, and Bernard Witholt. The time was ripe for a series of books on Pseudomonas because of its importance in human and plant pathogenesis, bio?lms, soil and rhizosphere colonization, etc. Efforts were devoted to produce the ?rst three volumes of the series on the biology of Pseudomonas after a meeting with Kluwer staff members in August 2002 during the XI IUMS conference in Paris (France). In less than a year a group of outstanding scientists in the ?eld, after devoting much of their valuable time, managed to complete their chapters for the three volumes of the series. To ensure the high standard of each chapter, renowned scientists participated in the reviewing process. The three books collected part of the "explosion" of new vital information on the genus Pseudomonas.

This book covers the wide set of well-regulated virulence factors and defense mechanisms of Pseudomonas aeruginosa focusing on stress responses and the evolution of this opportunistic human pathogen. Pseudomonas aeruginosa is responsible for one out of ten hospital infections. Additionally, this Gram-negative bacterium is accountable for persistent infections in immunocompromised individuals and the leading cause of chronic lung infections in cystic fibrosis patients. This book provides insight on the metabolic versatility of Pseudomonas aeruginosa and its mechanisms for biofilm formation that make this organism highly efficient in causing infections. The book invites the readers to learn more about the intrinsic ability of Pseudomonas aeruginosa to resist a wide variety of antimicrobial agents due to the concerted action of multidrug efflux pumps, antibiotic-degrading enzymes, and the low permeability of bacterial cellular envelopes. Particular focus is put on the evolutionary role of different types of protein-secretion systems in pathogenesis, flagella and their role in chemotaxis and surface sensing, and host-pathogen interactions. This book is a useful introduction to the field for junior scientists interested in the biology and pathogenesis of Pseudomonas aeruginosa. It is also an interesting read for advanced scientists and medical specialists working within this field, providing a broader view of the topic beyond their specific area of specialization.

Pseudomonas volume 7 collects some of the most relevant and emerging issues in the biology of these microorganisms, and a number of other important issues that were not collected in the previous volumes. The first six volumes of the Pseudomonas series covered the biology of pseudomonads in a wide range of contexts, including the niches they inhabit, the taxonomic relations among its members of this group, the molecular biology of gene expression in different niches and under different environmental conditions, the analysis of virulence in plants, animal and human pathogens, as well as the determinants that make some of these strains of interesting for biotechnological applications. This seventh volume covers the following topics: The history of the biology of Pseudomonas The use of Pseudomonas as biological agents New trends in the molecular biology of these microorganisms Pseudomonas and the immune system of insects and animals This book will be of use to researchers working on these bacteria, particularly those studying medical aspects of Pseudomonas, and their use as a means to control pathogens or to stimulate plant growth. This volume is also interesting for those studying the physiology, genetics, molecular biology of Pseudomonas and those using novel-omics approaches to understand bacteria of the genus Pseudomonas.

TwentyyearshavegonebysinceJackSokatch?rstpublishedhisoutsta- ingTheBiologyofPseudomonasbackin1986.Thiswasfollowedbytwobooks published by the ASM that contained the presentations of the Pseudomonas meetings held in Chicago in 1989 and Trieste in 1991. The earlier volume of these two was edited by Simon Silver, Al Chakrabarty, Barbara Iglewski, and Sam Kaplan, and the later one by Enrica Galli, Simon Silver, and Bernard Witholt. The time was ripe for a series of books on Pseudomonas because of its importance in human and plant pathogenesis, bio?lms, soil and rhizosphere colonization, etc. Efforts were devoted to produce the ?rst three volumes of the series on the biology of Pseudomonas after a meeting with Kluwer staff members in August 2002 during the XI IUMS conference in Paris (France). In less than a year a group of outstanding scientists in the ?eld, after devoting much of their valuable time, managed to complete their chapters for the three volumes of the series. To ensure the high standard of each chapter, renowned scientists participated in the reviewing process. The three books collected part of the "explosion" of new vital information on the genus Pseudomonas.

Pseudomonas comprises three volumes covering the biology of pseudomonads in a wide context, including the niches they inhabit, the taxonomic relations among members of this group, the molecular biology of gene expression in different niches and under different environmental conditions, the analysis of virulence traits in plants, animals and human pathogens as well as the determinants that make some strains useful for biotechnological applications and promotion of plant growth. There has been growing interest in pseudomonads and a particular urge to understand the biology underlying the complex metabolism of these ubiquitous microbes. These bacteria are capable of colonizing a wide range of niches, including the soil, the plant rhizosphere and phylosphere, and animal tissues; more recently they have attracted attention because of their capacity to form biofilms, a characteristic with potentially important medical and environmental implications. The three volumes cover the following topics: - Taxonomy, - Genomics, - Life styles, - Cell Architecture, - Virulence, - Regulation, - Macromolecules, - Alternative Respiratory Substrates, - Catabolism and Biotransformations. Pseudomonas will be of use to all researchers working on these bacteria, particularly those studying microbiology, plant crops, pathogenesis, and chemical engineering. Advanced students in biology, medicine and agronomy will also find these three volumes a valuable reference during their studies.

Pseudomonas comprises three volumes covering the biology of pseudomonads in a wide context, including the niches they inhabit, the taxonomic relations among members of this group, the molecular biology of gene expression in different niches and under different environmental conditions, the analysis of virulence traits in plants, animals and human pathogens as well as the determinants that make some strains useful for biotechnological applications and promotion of plant growth. There has been growing interest in pseudomonads and a particular urge to understand the biology underlying the complex metabolism of these ubiquitous microbes. These bacteria are capable of colonizing a wide range of niches, including the soil, the plant rhizosphere and phylosphere, and animal tissues; more recently they have attracted attention because of their capacity to form biofilms, a characteristic with potentially important medical and environmental implications. The three volumes cover the following topics: - Taxonomy, - Genomics, - Life styles, - Cell Architecture, - Virulence, - Regulation, - Macromolecules, - Alternative Respiratory Substrates, - Catabolism and Biotransformations. Pseudomonas will be of use to all researchers working on these bacteria, particularly those studying microbiology, plant crops, pathogenesis, and chemical engineering. Advanced students in biology, medicine and agronomy will also find these three volumes a valuable reference during their studies.

This volume collects new information on the genomics of saprophytic soil Pseudomonas, as well as functions related to genomic islands. It explores life styles in different settings and sheds further insights on the wide metabolic potential of this microbe for the removal of pollutants and production of added-value products. This volume also explores how Pseudomonas responds and reacts to environmental signals, including detection of cell density.