This up-to-date book compiles the basic and advanced laboratory techniques of mammalian cell culture. It is divided into four major sections encompassing the basics of cell culture, nucleic acid and protein isolation, cell-staining techniques, and cell transfection and single-cell analysis. The topics include aseptic handling, media preparation, and passaging of cells. The book also outlines downstream assays such as nucleic acid and protein isolation from in-vitro cell cultures. It provides elaborate stepwise protocols, key points, and troubleshooting tips. Key Features Provides step-wise protocols, flowcharts, key points to consider and troubleshooting tips Covers cellular staining using fluorescent dyes, genetic manipulation of cells via transfection, and an introduction to single-cell analyses Discusses basics in cell culture and downstream applications including gene and protein expression analysis Includes the principles underlying each of the techniques and provides a detailed methodology to practice Explores the whole range of techniques- from basic to downstream applications and advanced methods The book is essential for students and researchers in the field of life sciences, biotechnology, genetics, and molecular biology.