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This monograph focuses on modern femtosecond laser microscopes for
two photon imaging and nanoprocessing, on laser tweezers for cell
micromanipulation as well as on fluorescence lifetime imaging
(FLIM) in Life Sciences. The book starts with an introduction by
Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the
chapter on clinical multiphoton tomography, the novel high
resolution imaging technique. It includes a foreword by the
nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I:
Basics Brief history of fluorescence lifetime imaging The long
journey to the laser and its use for nonlinear optics Advanced
TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II:
Modern nonlinear microscopy of live cells STED microscopy:
exploring fluorescence lifetime gradients for super-resolution at
reduced illumination intensities Principles and applications of
temporal-focusing wide-field two-photon microscopy FLIM-FRET
microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen
sensing Laser tweezers are sources of two-photon effects Metabolic
shifts in cell proliferation and differentiation Femtosecond laser
nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue
imaging Multiphoton Tomography (MPT) Clinical multimodal CARS
imaging In vivo multiphoton microscopy of human skin Two-photon
microscopy and fluorescence lifetime imaging of the cornea
Multiscale correlative imaging of the brain Revealing interaction
of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM
in cosmetic clinical research Multiphoton microscopy and
fluorescence lifetime imaging for resection guidance in malignant
glioma surgery Non-invasive single-photon and multi-photon imaging
of stem cells and cancer cells in mouse models Bedside assessment
of multiphoton tomography
Nanostructuring of materials is a task at the heart of many modern
disciplines in mechanical engineering, as well as optics,
electronics, and the life sciences. This book includes an
introduction to the relevant nonlinear optical processes associated
with very short laser pulses for the generation of structures far
below the classical optical diffraction limit of about 200
nanometers as well as coverage of state-of-the-art technical and
biomedical applications. These applications include silicon and
glass wafer processing, production of nanowires, laser transfection
and cell reprogramming, optical cleaning, surface treatments of
implants, nanowires, 3D nanoprinting, STED lithography, friction
modification, and integrated optics. The book highlights also the
use of modern femtosecond laser microscopes and nanoscopes as novel
nanoprocessing tools.
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Paperback
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R398
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Discovery Miles 3 300
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