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Mast Cells: Methods and Protocols, Second Edition expands upon the
previous edition with current, detailed reviews that cover topics
of interest to mast cell neophytes and cognoscenti alike. With new
chapters on methods for the enumeration of tissue mast cells and
isolation of mature mast cells and mast cell progenitors from
mammalian tissues, Danio rerio (zebrafish) model in the study of
mast cell development and function, the functions of mast cells in
human health and disease and methods for the isolation, methods for
the investigation of the molecular mechanisms of mast cell
activation and their effector functions, assays for the detection
and analysis of mast cell secretory and cell surface phenotype and,
mast cell activation state, and experimental mouse models of
disease that have been deemed useful for the assessment of mast
cell functions in the regulation of innate and adaptive immune
response in cancer, tissue fibrosis, auto-inflammation and allergic
disease. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
cutting-edge, Mast Cells: Methods and Protocols, Second Edition
provides a sampler of useful methods and techniques in the further
study of mast cell biology.
Mast Cells: Methods and Protocols, Second Edition expands upon the
previous edition with current, detailed reviews that cover topics
of interest to mast cell neophytes and cognoscenti alike. With new
chapters on methods for the enumeration of tissue mast cells and
isolation of mature mast cells and mast cell progenitors from
mammalian tissues, Danio rerio (zebrafish) model in the study of
mast cell development and function, the functions of mast cells in
human health and disease and methods for the isolation, methods for
the investigation of the molecular mechanisms of mast cell
activation and their effector functions, assays for the detection
and analysis of mast cell secretory and cell surface phenotype and,
mast cell activation state, and experimental mouse models of
disease that have been deemed useful for the assessment of mast
cell functions in the regulation of innate and adaptive immune
response in cancer, tissue fibrosis, auto-inflammation and allergic
disease. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
cutting-edge, Mast Cells: Methods and Protocols, Second Edition
provides a sampler of useful methods and techniques in the further
study of mast cell biology.
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