This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro vided for individual analyses. The intention is to provide general guide lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of meth ods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations."

Wir sind umgeben von Kohlenhydraten: der susse Kaffee, Tee oder Dessert, die Starke als Hauptkomponente unserer Nahrung und die Zellulose als Strukturelement in Pflanzen. Kohlenhydrate sind eine wichtige Klasse biologischer Molekule, die an einer Anzahl wichtiger biochemischer Prozesse beteiligt sind. Gerade beginnen wir die Rolle von komplexen Zuckern zu verstehen, die an Proteine gebunden die Kommunikation von Zellen in einer "Zuckersprache" bewerkstelligen. Und nicht zuletzt kommen die ersten Kohlenhydratmolekule als Medikamente auf den Markt.
Anders als fur andere Biopolymere sind die analytischen Methoden zur strukturellen Charakterisierung und Sequenzanalyse fur Kohlenhydrate zur Zeit ungenugend, zum Teil wegen der uberwaltigenden Isotopenzahl der Zucker.
Dieses Buch beschreibt die Entwicklung der letzten Jahre, die mit der Kapillarelektrophorese in Bezug auf eine miniaturisierte Analytik mit besserer Auflosung und Empfindlichkeit gemacht wurden. Instrumentierung, Derivatisierung, Trennbedingungen und Anwendungen in verschiedenen Disziplinen und Industrien wie z. B. Glykobiologie, Lebensmittelindustrie und Biotechnologie werden beschrieben."

Dieser erste Titel einer ganzen Serie von anwendungsbezogenen Handbuchern zur Kapillarelektrophorese beschaftigt sich mit der Analytik von pharmazeutischen Substanzen. Dabei werden verschiedene Techniken praxisnah erlautert. Jeder, der im Labor - ob wissenschaftlich oder praxisnah - mit der Analyse von oft chiralen Pharmazeutika konfrontiert ist, wird viele Hinweise und Tips fur seine Arbeit finden.USP: Einzige Monographie zur Analyse von Pharmazeutika mit CE
This book describes the current state of the art for the analysis of pharmaceuticals by capillary electrophoresis and contains several hundred references to specific applications and methods. The main purpose of the book is to present the application possibilities of CE an therefore tabulated application data are provided.
Chapters of the book are devoted to providing details of individual application areas such as chiral analysis, determination of drug related impurities, determination of drug counter-ions, drug residue monitoring and main component assay. An introductory chapter provides theoretical background to CE an related techniques. A chapter is dedicated to capillary electrochromatography which highlights the importance this technique currently possesses. Successful regulatory acceptance of CE methods is also described. A comprehensive chapter covers method validation aspects. Other chapters include discrete areas such as the use of non-aqueous solvents, forensic applications of CE, the application of experimental designs, determination of drugs in biofluids, and the analysis of vitamins by CE."

The goal of this book is to show recent developments in the CE analysis of protein pharmaceuticals. It is devoted completely to practical concerns to strengthen the use of CE within the biotechnology industry, highlighting the uses of CE in various areas of product development including formulation studies, process development, product characterization and validated lot release and stability testing.

This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro vided for individual analyses. The intention is to provide general guide lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of meth ods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations."

Die Kapillarelektrophorese (CE) ist heute in biochemischen Laboratorien noch nicht als Routinemethode zur DNA-Trennung etabliert, obwohl sie diverse Vorzuge gegenuber den derzeitigen Methoden besitzt.
In diesem Band stellen verschiedene Wissenschaftler die theoretischen Aspekte der Trennung von Oligonucleinsauren mit der CE, die instrumentellen Grundlagen und deren Grenzen und schliesslich die praktische Anwendung in der Biochemie und Molekularbiologie vor.
This book on capillary electrophoresis is unique in its focus on the separation of nucleic acids. The importance of electrophoretic separation in every molecular biology laboratory justifies this specialization, which is also reflected in the development of instrumentation.
This book is aimed to help to implement this rather new and promising technology in the biological laboratories and to help to overcome typical problems that can occur when starting with a new technique. It should also trigger further development in the field. It covers the theoretical background as well as practical examples of usual applications.
The authors are all experts in their field, having many years of experience with capillary electrophoresis and their application to nucleic acids."