This book deals with current procedures for Practical Biochemistry. This is Kind of Work book. Revision exercises given in this book Helps Medical Students, non medical and, paramedical students as well. An Easy Approach to Practical Biochemistry Serves as self guide to the Students. Written By Good, Talented, Experienced Authors G. Amar Nagesh Kumar PhD Scholar Narayana Medical College, Nellore India Dr Robby Kumar PhD Lecturer, Department of Biochemistry SSR Medical College Mauritius. In this book latest laboratory diagnostic tests and few techniques in biochemistry department with basic principles are well described in simple language. I hope many medical, paramedical and other science undergraduate students will be benefited by this book.

A primer is a strand of nucleic acid that serves as a starting point for DNA replication. They are required because the enzymes that catalyze replication, DNA polymerases, can only add new nucleotides to an existing strand of DNA.The primers are to be designed correctly in thr amplification of a single DNA fragment corresponding to the target reagion of the template molecule.The primer should be complementary to its template strand at the 5' and 3' ends of the DNA region. in order for hybridization to occur . Amplification can occur when mismatching primers are close enough together on opposite strands of DNA - and an unwanted sequence is produced with ends that precisely match the primers. If such an 'incorrect' fragment is synthesized in the early cycles of a PCR, it will be efficiently amplified on subsequent cycles.