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This volume describes up-to-date techniques used in understanding the molecular biology of acute myeloid leukemia (AML) and addressing advances in diagnosis, classification, prognostication, and therapeutic strategies to potentially impact overall patient survival. The chapters in this book cover topics such as: cytochemical staining, single-cell mass cytometry of AML and Leukemia stem/progenitor cells, microsphere-based assessment of DNA methylation for AML prognosis, a zebrafish model for evaluating the function of human leukemic gene IDH1 and its mutation, and the isolation of biologically active exosomes from plasma of patients with cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Acute Myeloid Leukemia: Methods and Protocols is a valuable resource for scientists and researchers to further their studies and advancements in the field of AML.
Since the publication of Nonistopic DNA Probe Techniques in 1992,
the move away from radioactive materials for research and
diagnostics has continued. This is due in part to public awareness
of the hazards of radioactive waste and laws making radioactive
disposal more difficult and costly and to improvement in both the
sensitivity and convenience of nonisotopic techniques. Several new
nonisotopic techniques have been developed and substantial
improvements made to existing nonisotopic methods since 1992, and
these are now included in Nonisotopic Probing, Blotting, and
Sequencing.
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Paperback
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Discovery Miles 8 950
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