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Since the publication of Nonistopic DNA Probe Techniques in 1992,
the move away from radioactive materials for research and
diagnostics has continued. This is due in part to public awareness
of the hazards of radioactive waste and laws making radioactive
disposal more difficult and costly and to improvement in both the
sensitivity and convenience of nonisotopic techniques. Several new
nonisotopic techniques have been developed and substantial
improvements made to existing nonisotopic methods since 1992, and
these are now included in Nonisotopic Probing, Blotting, and
Sequencing.
Nonisotopic Probing, Blotting, and Sequencing is an updated,
expanded edition of the bestseller, Nonisotopic DNA Probe
Techniques. It has been thoroughly revised to include the latest
improvements in nonisotopic tagging techniques for macromolecules.
Like its predecessor, it enables researchers to select the best
nonisotopic method for their needs and maximize success by
following its straightforward protocols.
Key Features
* Provides strategies and detailed procedures for labeling,
blotting, and probing specific nucleic acid sequences and, with
this edition, protein molecules
* Gives protocols for nonisotopic DNA sequencing - new in this
edition
* Gives extensive, practical information
* Presents background information for each method
* Provides expert accounts from the inventor or developer of each
method
* Contains seven entirely new chapters
* Covers all major types of nonisotopic procedures for labeling and
detection
This volume describes up-to-date techniques used in understanding
the molecular biology of acute myeloid leukemia (AML) and
addressing advances in diagnosis, classification, prognostication,
and therapeutic strategies to potentially impact overall patient
survival. The chapters in this book cover topics such as:
cytochemical staining, single-cell mass cytometry of AML and
Leukemia stem/progenitor cells, microsphere-based assessment of DNA
methylation for AML prognosis, a zebrafish model for evaluating the
function of human leukemic gene IDH1 and its mutation, and the
isolation of biologically active exosomes from plasma of patients
with cancer. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
practical, Acute Myeloid Leukemia: Methods and Protocols is a
valuable resource for scientists and researchers to further their
studies and advancements in the field of AML.
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