The vascular tissue of higher plants has been an object of continuous detailed study since the invention of the light microscope. As relevant new physical, chemical and biochemical techniques and concepts have appeared over the years, they have regularly and immediately been applied to this field of enquiry. The reasons are not far to seek. Vascular tissue provides the long-distance transport system for water and nutrients within the plant, and an understanding of the structure and development of the pathways within it is essential for an under- standing of growth in all higher plants, including crop plants. Moreover, parts of the vascular tissue - the whole of the xylem and the fibers of the proble- have been, and still are, in high demand commercially for their unique properties and as the only renewable structural material on earth. There are, in addition, more subtle ways in which xylogenesis, particularly as it allows the development of tree species, contributes to the environment. A few years ago, while walking in the foothills of the Pyrenees, I found all this expressed more elegantly than I can express it, in a plea carved in wood at the entrance to a forest: Homme!! Je suis la chaleur de ton foyer par les froids nuits d'hiver, l'ombrage ami lorsque brule Ie soleil d'ete, l'eau des rivieres et des sources. Je suis la charpente de ta maison, la planche de ta table.

In this third edition the authors have written a guide to the essentials of plant tissue culture. The book takes the reader through a graded series of experimental protocols and provides an introductory review of each topic. There are discussions of aseptic techniques, and nutritional components of media. Subsequent chapters cover such topics as callus formation, organogenesis, xylem cell differentiation, root cultures, cell suspensions, micropropagation by buds, somatic embryogenesis, protoplast culture and fusion, secondary metabolite production, and quantitation of procedures. A glossary, commercial sources of supplies, and the formulations of culture media are also included. This new edition has been completely revised and updated. The organization has been improved, and new illustrations have been added, together with new experiments on such topics as potato callus formation, and embryo culture. This book will be useful to graduate students and professionals in plant science, forestry, agronomy, and horticulture.