It is increasingly clear that signal transduction is a highly organized and integrated process. Divided into two convenient sections, Signal Transduction Protocols, Third Edition focuses on experimental approaches to better understand the complexity of signal transduction. Introductory chapters provide perspective on several of the challenges involved in signal transduction research and offer guidance on selecting the best approaches to various types of questions. The individual chapters provide detailed experimental protocols covering various topics, from the effects of ligand binding on receptor conformation and effector coupling, to moving inside the cell in order to capture the spatial and temporal characteristics of signaling events. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Signal Transduction Protocols serves as an ideal guide to scientists of all backgrounds and is a valuable resource for future progress in the field of signal transduction research.

It is increasingly clear that signal transduction is a highly organized and integrated process. Divided into two convenient sections, Signal Transduction Protocols, Third Edition focuses on experimental approaches to better understand the complexity of signal transduction. Introductory chapters provide perspective on several of the challenges involved in signal transduction research and offer guidance on selecting the best approaches to various types of questions. The individual chapters provide detailed experimental protocols covering various topics, from the effects of ligand binding on receptor conformation and effector coupling, to moving inside the cell in order to capture the spatial and temporal characteristics of signaling events. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Signal Transduction Protocols serves as an ideal guide to scientists of all backgrounds and is a valuable resource for future progress in the field of signal transduction research.

This special volume of "Progress in Molecular Biology and Translational Science "focuses on the molecular biology of arrestins, with contributions from leaders in the field. Arrestins have emerged as central players in the regulation of many facets of G protein-coupled receptor signaling. This volume covers a variety of topics with reviews written by experts in the field.

Key features:

* Contributions from leading authorities * Informs and updates on all the latest developments in the field