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This volume details computational techniques for analyses of a wide
range of biological contexts, providing an overview of the most
up-to-date techniques used in the field. Chapters guide the reader
through available data resources and analysis methods and
easy-to-follow protocols that allow the researcher to apply various
computational tools to an array of different data types. Written in
the highly successful Methods in Molecular Biology series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory and computational protocols, and tips on
troubleshooting and avoiding known pitfalls. Authoritative and
cutting-edge, Computational Cell Biology: Method and Protocols aims
to ensure successful results in the further study of this vital
field.
This second edition provides up-to-date protocols focusing on
techniques for the specific enrichment of phosphopeptides and
phosphoproteins. Phosphoproteomics Methods and Protocols, Second
Edition guides readers through different labeling strategies for
quantitative phosphoproteomics; high-throughput mass
spectrometry-based phosphoproteome analyses and phospho flow
cytometry; and bioinformatics strategies for phosphoproteomics data
analysis and integration. Additional protocols concentrate on the
identification of kinase-substrate relationships by both high- and
low-throughput approaches. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions
to their respective topics, lists of the necessary materials and
reagents, readily reproducible laboratory protocols, and key tips
on troubleshooting and avoiding known pitfalls. Authoritative and
practical, Phosphoproteomics Methods and Protocols, Second Edition
aims to ensure successful results in the further study of this
vital field.
This book comprises protocols describing systems biology
methodologies and computational tools, offering a variety of ways
to analyze different types of high-throughput cancer data. Chapters
give an overview over data types available in large-scale data
repositories and state-of-the-art methods used in the field of
cancer systems biology. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cancer Systems Biology : Methods and
Protocols aims to ensure successful results in the further study of
this vital field. The chapters "Identifying Genetic Dependencies in
Cancer by Analyzing siRNA Screens in Tumor Cell Line Panels",
"Perseus: A Bioinformatics Platform for Integrative Analysis of
Proteomics Data in Cancer Research" and "Phosphoproteomics-based
Profiling of Kinase Activities in Cancer Cells" are available open
access under a CC BY 4.0 license via link.springer.com.
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