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Genetic diversity was assessed using 15 morphological characters. Based on multivariate (D2) analysis, 60 mango genotypes were grouped into 8 clusters. Three enzymes viz., gulutamate oxaloacetate transminase (GOT), malate dehydrogenase (MDH) and peroxidase (PER) were used to investigate genetic diversity of 60 mango genotypes at protein level. Eight zymotypes with GOT, 10 with MDH and 7 with PER were formed by 22, 39, 12 bands, respectively at different Rf values. Compared with three studied DNA extraction protocols of mango such as SDS, CTAB and water saturated ether (WSE) method with NaCl, it was found that WSE method with NaCl had highest value of average percentage (85.44%) in DNA content of the mango genotypes. Study of RAPD for 60 mango genotypes was done to detect genetic diversity at DNA level. Of 40 primers, 11 decamer primers amplified 104 bands. The mean genetic diversity among all the accessions was 0.323 and Shannon's information index was 0.489 across all loci. Induction of mutation on 8 selected genotypes derived from morph-molecular diversity study was performed by applying three doses of radiation such as 20 Gy, 30 Gy and 40 Gy.
This book is prepared on the basis of some genetical, breeding, tissue culture aspects of an important oil yielding crop Groundnut. These different aspects are done by both field and laboratory experiments. The potential of a crop to respond favorably to breeding programme depends upon the nature and magnitude of variability. Groundnut production can be increased through breeding research following development of high yielding drought tolerant genotypes, selection of superior genotypes, and creating genetic variability in the most adaptive cultivars. Correlation studies provide reliable and useful information on the nature, extent and direction of selection. Genetic divergence is essential to select the parents for future breeding program. Biotechnological approaches including tissue culture can play an important role in creating diversities and developing new varieties. All of these are shown and discussed by actual field and laboratory data here. This book will serve as a poteltial book for the students and researchers of breeding, genetics or even biotechnology.
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