The Handbook of Plant Ecophysiology Techniques you have now in your hands is the result of several combined events and efforts. The birth of this handbook can be traced as far as 1997, when our Plant Ecophysiology lab at the University of Vigo hosted a practical course on Plant Ecophysiology Techniques. That course showed us how much useful a handbook presenting a bunch of techniques would be for the scientists beginning to work on Plant Ecophysiology. In fact, we wrote a short handbook explaining the basics of the techniques taught in that 1997 course: Flow cytometry to measure ploidy levels, Use of a Steady-State porometer to measure transpiration, In vivo measure of fluorescence, HPLC analysis of low molecular weight phenolics, Spectrophotometric determinations of free proline and soluble proteins, TLC polyamines contents measures, Isoenzymatic electrophoresis, Use of IRGA and oxygen electrode. That modest handbook, written in Spanish, was very helpful, both for the people who attended the course and for other who have used it for beginning to work in Plant Ecophysiology. The present Handbook is much more ambitious, and it includes more techniques. But we have also had in mind the young scientists beginning to work on Plant Ecophysiology. In 1999 Francois Pellissier leaded a proposal presented to the European Commission in the Fifth Framework Program in the High Level * Scientific Conferences, including three EuroLab Courses about lab and field techniques useful to improve allelopathic research."

The rapidly growing human population has increased the dependence on fossil fuel based agrochemicals such as fertilizers and pesticides to produce the required agricultural and forestry products. This has exerted a great pressure on the non renewable fossil fuel resources, which cannot last indefinitely. Besides, indiscriminate use ofpesticides for pests (weeds, insects, nematodes, pathogens) control has resulted in serious ecological and environmental problems viz., (A) Increasing incidence of resistance in pest organisms to important pesticides. (B) Shift in pests population, particulary in weeds and insects. In weeds, species that are more closely related to the crops they infest have developed. In insects, scenario is most grim, the predators have been killed and minor insect pests have become major pests and require very heavy doses ofhighly toxic insecticides for their control. (C) Greater environmental pollution and health hazards (a) particularly from contamination of surface and underground drinking water resources and (b) from their inhalation during handling and application. (D) Toxic residues of pesticides pollute the environment and may prove hazardous to even our future generations. (E) Some agricultural commodities may contain minute quantities ofpesticides residues, with long tenn adverse effects on human and livestock health. Therefore, serious ecological questions about the reliance on pesticides for pests control has been raised. The use of fertilizers, besides causing environmental problems has also impoverished the soil health and decreased the beneficial soil fauna. For example, in some major crop rotations viz."

The rapidly growing human population has increased the dependence on fossil fuel-based agrochemicals, such as fertilizers and pesticides, to produce the required agricultural and forestry products. This has exerted great pressure on non-renewable fossil fuel resources, which cannot last indefinitely. Not only do agrochemicals pollute the environment, but pests also become resistant to pesticides. Thus, present agricultural practices exploit natural resources, and damage fauna and flora and agroecosystems. One safe alternative to overcome these problems is the use of allelopathy to sustain development in agriculture and forestry and maintain a clean environment for future generations. This book is the Proceedings of the III International Congress on Allelopathy in Ecological Agriculture and Forestry, held on August 18-21, 1998, at the University of Agricultural Sciences, Dharwad, Karnataka, India, and provides an updated status of current allelopathy research in various leading countries, with the overall aim of developing new technologies for ecological agriculture and forestry in the 21st century. To date, no book on ecological agriculture has discussed these aspects, hence it is the first time that such information is available. The chapter contributors are leading specialists in their fields, and all chapters have been peer-reviewed by international referees. This book will be indispensable for agricultural scientists (agronomists, entomologists, nematologists, plant pathologists, horticulturists, plant breeders, agroforesters, foresters, soil scientists), bioscientists (biochemists, organic chemists, plant ecologists, microbiologists and limnologists), environmentalists, graduate students and farmers, as well as for organizations engaged in sustainable agriculture and organic agriculture.

The Handbook of Plant Ecophysiology Techniques you have now in your hands is the result of several combined events and efforts. The birth of this handbook can be traced as far as 1997, when our Plant Ecophysiology lab at the University of Vigo hosted a practical course on Plant Ecophysiology Techniques. That course showed us how much useful a handbook presenting a bunch of techniques would be for the scientists beginning to work on Plant Ecophysiology. In fact, we wrote a short handbook explaining the basics of the techniques taught in that 1997 course: Flow cytometry to measure ploidy levels, Use of a Steady-State porometer to measure transpiration, In vivo measure of fluorescence, HPLC analysis of low molecular weight phenolics, Spectrophotometric determinations of free proline and soluble proteins, TLC polyamines contents measures, Isoenzymatic electrophoresis, Use of IRGA and oxygen electrode. That modest handbook, written in Spanish, was very helpful, both for the people who attended the course and for other who have used it for beginning to work in Plant Ecophysiology. The present Handbook is much more ambitious, and it includes more techniques. But we have also had in mind the young scientists beginning to work on Plant Ecophysiology. In 1999 Francois Pellissier leaded a proposal presented to the European Commission in the Fifth Framework Program in the High Level * Scientific Conferences, including three EuroLab Courses about lab and field techniques useful to improve allelopathic research."