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The book deals with the theory and practice of all electrophoretic
steps leading to proteome analysis, i.e. isoelectric focusing
(including immobilized pH gradients), sodium dodecyl sulphate
electrophoresis (SADS-PAGE) and finally two-dimensional maps. It is
a reasoned collection of all modern, relevant, up-to-date
methodologies leading to successful fractionation, analysis and
characterization of every polypeptide spot in 2-D map analysis. It
includes chapters on the most sophisticated mass spectrometry
developments and it helps the reader in navigating through the most
important databases in proteome analysis, including step by step
tours in selected sites. Yet, this book's unique strength and
feature is the fact that it combines not only practice (in common
with any other book on this topic) but also theory, by giving a
detailed treatment on the most advanced theoretical treatments of
steady-state techniques, such as isoelectric focusing and
immobilized pH gradients. A lot of this theory is newly developed
and presented to the public for the first time. Thus, this book
should satisfy not only the needs of every day practitioners, but
also the desires of the most advanced theoreticians in the field,
who will surely appreciate the novel theories presented here.
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