This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.

The 2015 Paris Agreement represents the culmination of years of intense negotiations under the United Nations Framework Convention on Climate Change. Designed to curb climate change, it was negotiated by almost 200 countries who came to the table with different backgrounds, perceptions and interests. As such, the Agreement represents a triumph for multilateralism in a period otherwise characterized by nationalist turns. How did countries reach the historical agreement, and what were the driving forces behind it? This book paints a full picture by providing and analysing multifaceted insider accounts from high-level delegates who represented developed and developing countries, civil society, businesses, the French Presidency, and the UNFCCC Secretariat. In doing so, the book documents not only the negotiation of the Paris Agreement but also the dynamics and factors that shaped it. A better understanding of these dynamics and factors can guide future negotiations and help us solve global challenges.

The 2015 Paris Agreement represents the culmination of years of intense negotiations under the United Nations Framework Convention on Climate Change. Designed to curb climate change, it was negotiated by almost 200 countries who came to the table with different backgrounds, perceptions and interests. As such, the Agreement represents a triumph for multilateralism in a period otherwise characterized by nationalist turns. How did countries reach the historical agreement, and what were the driving forces behind it? This book paints a full picture by providing and analysing multifaceted insider accounts from high-level delegates who represented developed and developing countries, civil society, businesses, the French Presidency, and the UNFCCC Secretariat. In doing so, the book documents not only the negotiation of the Paris Agreement but also the dynamics and factors that shaped it. A better understanding of these dynamics and factors can guide future negotiations and help us solve global challenges.

The Middle East and North Africa region has been plagued with civil wars, international interventions, and increasing militarization, making it one of the most war-affected areas in the world today. Despite numerous mediation processes and initiatives for conflict resolution, most have failed to transform conflicts from war to peace. Seeking to learn from these past efforts and apply new research, Fraihat and Svensson present the first comprehensive approach to mediation in the Arab world, taking on cases from Yemen to Sudan, from Qatar to Palestine, Syria, and beyond. Conflict Mediation in the Arab World focuses on mediation at three different levels of analysis: between countries, between governments and armed actors inside single countries, and between different communities. In applying this holistic method, the editors identify similarities and differences in the conditions for conflict resolution and management. Drawing upon the work of experts in the field with a deep understanding of the increasing complexities and changing dynamics of the region, this volume offers a valuable resource for academics, policy makers, and practitioners interested in conflict resolution and management in the Middle East and North Africa.

The Middle East and North Africa region has been plagued with civil wars, international interventions, and increasing militarization, making it one of the most war-affected areas in the world today. Despite numerous mediation processes and initiatives for conflict resolution, most have failed to transform conflicts from war to peace. Seeking to learn from these past efforts and apply new research, Fraihat and Svensson present the first comprehensive approach to mediation in the Arab world, taking on cases from Yemen to Sudan, from Qatar to Palestine, Syria, and beyond. Conflict Mediation in the Arab World focuses on mediation at three different levels of analysis: between countries, between governments and armed actors inside single countries, and between different communities. In applying this holistic method, the editors identify similarities and differences in the conditions for conflict resolution and management. Drawing upon the work of experts in the field with a deep understanding of the increasing complexities and changing dynamics of the region, this volume offers a valuable resource for academics, policy makers, and practitioners interested in conflict resolution and management in the Middle East and North Africa.