Although the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field.
Key Features
* Comprehensive protocols included for
* Detection of NO and related compounds by chemical, immunohistochemical, and "in situ" hybridization techniques
* Newly developed methods for the purification of neuronal and endothelial NO synthase, production of monoclonal antibodies to NO synthase, molecular cloning and expression of NO synthesis, and control of NO synthase gene expression
* Assessment of NO-mediated functions in neurons, central nervous system, cerebral circulation, synaptic transmission, and vascular tone
* Calcium imaging by confocal microscopy, evaluation of the effect of NO on iron metabolism, and detection of heme oxygenase-1 and -2 message level and distribution

his book provides a comprehensive summary of data from basic research on characterization, regulation, and function of heme oxygenase in mammalian systems. The book also includes a major section that covers the currently used clinical methods to suppress neonatal jaundice with emphasis on the newly developed use of synthetic metalloporophyrins. This book will be welcomed by researchers and students in pharmacology, biochemistry, pharmacy, neonatology, hematology, internal medicine, and endocrinology.