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This is an extensively illustrated laboratory manual of
transmission electron microscopy techniques for the technician,
graduate student, or researcher. Chapters begin with a general
discussion, move on to the chemicals and equipment required for the
method being described and conclude with a step-by-step
presentation of the method and instructions for the preparation of
solutions. Notes at the end of each chapter warn of possible
pitfalls and outline 'tricks of the trade'. The methods and
techniques outlined have been tested for over ten years in clinical
and research laboratory situations, and are entirely reliable.
Practical Electron Microscopy covers fixation, dehydration and
embedding, semi-thin and thin sectioning, the electron microscope,
and photography. For this new edition, the chapters on photography
and the electron microscope have been completely rewritten and two
new chapters have been added, one on immuno electron microscopy
using colloidal gold, and one dealing with such special techniques
as retrieving specimens from paraffin and handling nasal brushings
and blood samples. This manual will be an invaluable guide to
anyone using electron microscopy on human and animal tissue and
wishing to develop a routine that guarantees good and reproducible
results.
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