Mannich bases are very important and constructive compounds in organic synthesis, especially their role in biochemistry and natural products is of capital importance. A lot of work is going on over the synthesis, characterization and investigation of bioactivity of these compounds. In The present communication some novel Mannich bases derived from various aromatic aldehydes and aromatic amines with 2- butanone are reported with their biological role. The surprising spectra results showed some Schiff base formation along with the Mannich bases and this eventually establish a new method for the preparation of Schiff bases through acid catalyzed reaction first time. The novelty and biological role of these Mannich and Schiff bases are also reported here in this communication.