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Microbial keratitis is a serious ocular infection. It is often
difficult to establish a microbial keratitis that is due to fungal
infection. Inadequate therapy and delayed diagnosis of fungal
keratitis leads to generally poor visual outcomes. The objective of
this study is to determine the sensitivity and specificity of PCR
to detect fungal pathogen in microbial keratitis. In this study, a
PCR method was compared to the conventional method to detect fungal
pathogen in microbial keratitis. The PCR was performed with primers
pair F1, R2 and F3, R4 from two consecutive regions in the 18S rRNA
gene. Positive result for PCR was considered when there was
presence of this amplified target sequence in both primer pairs F1,
R2 and F3, R4. A statistical diagnostic test revealed that the PCR
has a sensitivity of 90.9% and a specificity of 94.7% to detect
fungal pathogen in microbial keratitis. PCR is a rapid, sensitive
and a useful method to detect fungal pathogen in microbial kerati
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