This book includes basics of impedance spectroscopy technology, substrate compatibility issues, integration capabilities, and several applications in detection of different analytes. It helps explore the importance of this technique in biological detection, related micro/nano-fabricated platforms and respective integration, biological synthesis schemes to carry out the detection, associated challenges, and related future directions. The various qualitative/quantitative findings of several modules are summarized in the form of the detailed descriptions, schematics, and tables. Features: Serves as a single source for exploring underlying fundamental principles and the various biological applications through impedance spectroscopy. Includes chapters based on non-biological applications of impedance spectroscopy and IoT enabled impedance spectroscopy-based methods for detection. Discusses derivations, substrates, applications, and several integrations. Describes micro/nanofabrication of impedance-based biological sensors. Reviews updated integrations like digital manufacturing and IoT. This book is aimed at researchers and graduate students in material science, impedance spectroscopy and biosensing.