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This monograph focuses on modern femtosecond laser microscopes for
two photon imaging and nanoprocessing, on laser tweezers for cell
micromanipulation as well as on fluorescence lifetime imaging
(FLIM) in Life Sciences. The book starts with an introduction by
Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the
chapter on clinical multiphoton tomography, the novel high
resolution imaging technique. It includes a foreword by the
nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I:
Basics Brief history of fluorescence lifetime imaging The long
journey to the laser and its use for nonlinear optics Advanced
TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II:
Modern nonlinear microscopy of live cells STED microscopy:
exploring fluorescence lifetime gradients for super-resolution at
reduced illumination intensities Principles and applications of
temporal-focusing wide-field two-photon microscopy FLIM-FRET
microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen
sensing Laser tweezers are sources of two-photon effects Metabolic
shifts in cell proliferation and differentiation Femtosecond laser
nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue
imaging Multiphoton Tomography (MPT) Clinical multimodal CARS
imaging In vivo multiphoton microscopy of human skin Two-photon
microscopy and fluorescence lifetime imaging of the cornea
Multiscale correlative imaging of the brain Revealing interaction
of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM
in cosmetic clinical research Multiphoton microscopy and
fluorescence lifetime imaging for resection guidance in malignant
glioma surgery Non-invasive single-photon and multi-photon imaging
of stem cells and cancer cells in mouse models Bedside assessment
of multiphoton tomography
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