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This comprehensive and unique text presents a full overview of
downstream processing useful for those new to the concept as well
as professionals with experience in the area. The history and
theoretical principles of Aqueous Two-Phase Systems (ATPS) are
covered in depth. Information on ATPS characterization and
application is included, and ATPS equilibria and system parameters
that have significant effect on partition behavior are studied.
Aqueous Two-Phase Systems for Bioprocess Development for the
Recovery of Biological Products addresses specific applications of
ATPS for the recovery and partial purification of high molecular
weight compounds such as proteins, nucleic acids and
polysaccharides, particulate bioproducts such as cells and
organelles and low molecular weight compounds. Non-conventional
strategies involving ATPS such as affinity systems, continuous
liquid-liquid fractionation stages and the recovery from plant
extracts are presented. Economic analysis of the application of
ATPS in comparison to other fractionation techniques, particularly
liquid chromatography, is considered, as are opportunity and
current trends in the ATPS research area. Each chapter utilizes the
contributors' experimental expertise in traditional and
non-conventional ATPS strategies, as well as analysis of areas of
opportunity and perspectives on the development and future
applications of ATPS in both the lab and larger scale operations.
The result is a thorough and singular overview of ATPS which has
not been matched by any other text on the market.
This comprehensive and unique text presents a full overview of
downstream processing useful for those new to the concept as well
as professionals with experience in the area. The history and
theoretical principles of Aqueous Two-Phase Systems (ATPS) are
covered in depth. Information on ATPS characterization and
application is included, and ATPS equilibria and system parameters
that have significant effect on partition behavior are studied.
Aqueous Two-Phase Systems for Bioprocess Development for the
Recovery of Biological Products addresses specific applications of
ATPS for the recovery and partial purification of high molecular
weight compounds such as proteins, nucleic acids and
polysaccharides, particulate bioproducts such as cells and
organelles and low molecular weight compounds. Non-conventional
strategies involving ATPS such as affinity systems, continuous
liquid-liquid fractionation stages and the recovery from plant
extracts are presented. Economic analysis of the application of
ATPS in comparison to other fractionation techniques, particularly
liquid chromatography, is considered, as are opportunity and
current trends in the ATPS research area. Each chapter utilizes the
contributors' experimental expertise in traditional and
non-conventional ATPS strategies, as well as analysis of areas of
opportunity and perspectives on the development and future
applications of ATPS in both the lab and larger scale operations.
The result is a thorough and singular overview of ATPS which has
not been matched by any other text on the market.
This book offers comprehensive information on all aspects of ELISA,
starting with the fundamentals of the immune system. It also
reviews the history of analytical assays prior to the advent of
ELISA (enzyme-linked immunosorbent assay) and addresses the
materials of choice for the fabrication of the platforms, possible
biomolecular interactions, different protocols, and evaluation
parameters. The book guides readers through the respective steps of
the analytical assay, while also familiarizing them with the
possible sources of error in the assay. It offers detailed insights
into the immobilization techniques used for protein attachment, as
well as methods for evaluating the assay and calculating the key
parameters, such as sensitivity, specificity, accuracy and limit of
detection. In addition, the book explores the advantages and
shortcomings of the conventional ELISA, as well as various
approaches to improving its performance. In this regard, merging
and integrating other technologies with widely known ELISAs have
opened new avenues for the advancement of this immunoassay.
Accordingly, the book provides cutting-edge information on
integrated platforms such as ELISpot, plasmonic ELISAs,
sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in
micro-devices.
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