To foster a better understanding of dopamine receptor functionality, this detailed volume creates an interface between updated classical methods and new emerging technologies heretofore not available to new or seasoned researchers. Divided in five sections dedicated to experimental approaches investigating different facets of dopaminergic signal transduction, Dopamine Receptor Technologies covers epigenetic and post-transcriptional analysis, computational and biochemical techniques, visualization and imaging methods, molecular and cell biological tools, as well as behavioral assessment. The book, as a part of the popular Neuromethods series, provides insightful step-by-step protocols and methodological reviews that readers will find useful. Practical and versatile, Dopamine Receptor Technologies seeks to aid researchers in developing new pharmacological tools to improve our knowledge of in vivo roles played by each receptor subtype and the synthesis of prospective lead compounds for drug discovery.

This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs. Sections explore the tweaking of ligands, bioluminescence and FRET approaches, specific GPCR signaling properties, as well as visualization of subcellular compartmentalization. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, G Protein-Coupled Receptor Signaling: Methods and Protocols serves as an ideal reference for life scientists working in a variety of research fields including molecular pharmacology, cell and developmental biology, brain behavior and physiology, drug development and screening. Chapter 4 is available open access under a CC BY 4.0 license via link.springer.com.

The fundamental role of Great Britain's economy in the international economic system in the century preceding the First World War is demonstrated by a number of variables, which have drawn the interest of many scholars. The focus here is on capital flows. The main difficulty encountered in this work arose from a shortage of documentation on economic data in the historical period under consideration, which has been tentatively reconstructed, on the basis of a number of estimates, subjected to a close comparative scrutiny. The book provides a valid guide to anyone wishing to improve their understanding of the so-called "pax britannica" which, at that time, rested on the canons of free trade and the gold standard. This historical period is considered by many to be the first experience of capitalist globalization. In this sense the book is also intended to provide useful reading for those who want to reflect on the possible future evolution of the world economy.

The fundamental role of Great Britain's economy in the international economic system in the century preceding the First World War is demonstrated by a number of variables, which have drawn the interest of many scholars. The focus here is on capital flows. The main difficulty encountered in this work arose from a shortage of documentation on economic data in the historical period under consideration, which has been tentatively reconstructed, on the basis of a number of estimates, subjected to a close comparative scrutiny. The book provides a valid guide to anyone wishing to improve their understanding of the so-called "pax britannica" which, at that time, rested on the canons of free trade and the gold standard. This historical period is considered by many to be the first experience of capitalist globalization. In this sense the book is also intended to provide useful reading for those who want to reflect on the possible future evolution of the world economy.

To foster a better understanding of dopamine receptor functionality, this detailed volume creates an interface between updated classical methods and new emerging technologies heretofore not available to new or seasoned researchers. Divided in five sections dedicated to experimental approaches investigating different facets of dopaminergic signal transduction, Dopamine Receptor Technologies covers epigenetic and post-transcriptional analysis, computational and biochemical techniques, visualization and imaging methods, molecular and cell biological tools, as well as behavioral assessment. The book, as a part of the popular Neuromethods series, provides insightful step-by-step protocols and methodological reviews that readers will find useful. Practical and versatile, Dopamine Receptor Technologies seeks to aid researchers in developing new pharmacological tools to improve our knowledge of in vivo roles played by each receptor subtype and the synthesis of prospective lead compounds for drug discovery.