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This volume explores various approaches for enrichment, detection,
isolation, and molecular profiling of circulating tumor cells
(CTCs). Each chapter provides comprehensive descriptions and
guidelines on how to perform innovative experiments in CTC
research. Included are protocols for capture of CTCs via filtration
and density gradient centrifugation; microfluidic and
immunomagnetic separation of CTCs; detection of CTCs by
immunocytochemistry, fluorescence in situ hybridization, and flow
cytometry; assays designed for genomic characterization and
functional analyses of CTCs, and many more. Written in the highly
successful Methods in Molecular Biology series format, the chapters
in this book include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls. Cutting-edge and
authoritative, Circula ting Tumor Cells: Methods and Protocols is a
valuable resource for laboratory researchers and clinicians who are
interested in furthering their studies on CTCs.
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