In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. Lipid Signaling Protocols, Second Edition guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the technical considerations, challenges and potential pitfalls associated with using these laboratory-based approaches. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lipid Signaling Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

The DSM-5 Alternative Model for Personality Disorders reviews and advances this innovative and increasingly popular scheme for diagnosing and evaluating personality disorders. The authors identify the multiple clinical, theoretical, and research paradigms that co-exist in the Alternative Model for Personality Disorders (AMPD) and show how the model can aid the practicing mental health professional in evaluating and treating patients as well as its importance in stimulating research and theoretical understanding of this domain. This work explores and summarizes methods of personality assessment and psychiatric evaluation, research findings, and clinical applications of the AMPD, highlighting its usefulness to clinical teaching and supervision, forensic application, and current research. It is a go-to reference for experienced professionals and researchers, those who wish to learn this new diagnostic system, and for clinicians in training.

In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. Lipid Signaling Protocols, Second Edition guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the technical considerations, challenges and potential pitfalls associated with using these laboratory-based approaches. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lipid Signaling Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

The DSM-5 Alternative Model for Personality Disorders reviews and advances this innovative and increasingly popular scheme for diagnosing and evaluating personality disorders. The authors identify the multiple clinical, theoretical, and research paradigms that co-exist in the Alternative Model for Personality Disorders (AMPD) and show how the model can aid the practicing mental health professional in evaluating and treating patients as well as its importance in stimulating research and theoretical understanding of this domain. This work explores and summarizes methods of personality assessment and psychiatric evaluation, research findings, and clinical applications of the AMPD, highlighting its usefulness to clinical teaching and supervision, forensic application, and current research. It is a go-to reference for experienced professionals and researchers, those who wish to learn this new diagnostic system, and for clinicians in training.