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PCR methods for the detection of microbial pathogens have made
relatively little impact in diagnostic microbiology laboratories
due to the common decision to use expensive commercially produced
tests rather than the cheaper alternative of developing one's own
tests or introducing tests developed by other workers. PCR
Detection of Microbial Pathogens, Second Edition presents
alternatives to commercially produced PCR methods to detect
microbial pathogens. Although most of the chapters in this book are
devoted to the detection of specific pathogens, the first chapters
in this book should appeal to anyone working in this field
regardless of their particular interests. Although PCR tests can
often be made to work with relatively little effort, it is often
unclear how efficient the PCR test is, how inhibitory the specimen
containing the pathogen of interest is and how the test can be
quality controlled. All of which are of great importance in
developing tests for diagnostic use. These topics are covered in
great depth at the beginning of the book. The main part of the book
is devoted to describing methods for the detection of a wide range
of pathogens and from widely different specimens and situations.
Written in the highly successful Methods in Molecular Biology (TM)
series format, chapters contain introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls. Authoritative and
accessible, PCR Detection of Microbial Pathogens, Second Edition
serves microbiologists regardless of their particular interest
because, when used together with the general principles, the sheer
variety of procedures provided here enables the reader to design
and introduce diagnostic tests in the laboratory with confidence.
PCR methods for the detection of microbial pathogens have made
relatively little impact in diagnostic microbiology laboratories
due to the common decision to use expensive commercially produced
tests rather than the cheaper alternative of developing one's own
tests or introducing tests developed by other workers. PCR
Detection of Microbial Pathogens, Second Edition presents
alternatives to commercially produced PCR methods to detect
microbial pathogens. Although most of the chapters in this book are
devoted to the detection of specific pathogens, the first chapters
in this book should appeal to anyone working in this field
regardless of their particular interests. Although PCR tests can
often be made to work with relatively little effort, it is often
unclear how efficient the PCR test is, how inhibitory the specimen
containing the pathogen of interest is and how the test can be
quality controlled. All of which are of great importance in
developing tests for diagnostic use. These topics are covered in
great depth at the beginning of the book. The main part of the book
is devoted to describing methods for the detection of a wide range
of pathogens and from widely different specimens and situations.
Written in the highly successful Methods in Molecular Biology (TM)
series format, chapters contain introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls. Authoritative and
accessible, PCR Detection of Microbial Pathogens, Second Edition
serves microbiologists regardless of their particular interest
because, when used together with the general principles, the sheer
variety of procedures provided here enables the reader to design
and introduce diagnostic tests in the laboratory with confidence.
This is an EXACT reproduction of a book published before 1923. This
IS NOT an OCR'd book with strange characters, introduced
typographical errors, and jumbled words. This book may have
occasional imperfections such as missing or blurred pages, poor
pictures, errant marks, etc. that were either part of the original
artifact, or were introduced by the scanning process. We believe
this work is culturally important, and despite the imperfections,
have elected to bring it back into print as part of our continuing
commitment to the preservation of printed works worldwide. We
appreciate your understanding of the imperfections in the
preservation process, and hope you enjoy this valuable book.
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