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PCR methods for the detection of microbial pathogens have made
relatively little impact in diagnostic microbiology laboratories
due to the common decision to use expensive commercially produced
tests rather than the cheaper alternative of developing one's own
tests or introducing tests developed by other workers. PCR
Detection of Microbial Pathogens, Second Edition presents
alternatives to commercially produced PCR methods to detect
microbial pathogens. Although most of the chapters in this book are
devoted to the detection of specific pathogens, the first chapters
in this book should appeal to anyone working in this field
regardless of their particular interests. Although PCR tests can
often be made to work with relatively little effort, it is often
unclear how efficient the PCR test is, how inhibitory the specimen
containing the pathogen of interest is and how the test can be
quality controlled. All of which are of great importance in
developing tests for diagnostic use. These topics are covered in
great depth at the beginning of the book. The main part of the book
is devoted to describing methods for the detection of a wide range
of pathogens and from widely different specimens and situations.
Written in the highly successful Methods in Molecular Biology (TM)
series format, chapters contain introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls. Authoritative and
accessible, PCR Detection of Microbial Pathogens, Second Edition
serves microbiologists regardless of their particular interest
because, when used together with the general principles, the sheer
variety of procedures provided here enables the reader to design
and introduce diagnostic tests in the laboratory with confidence.
PCR methods for the detection of microbial pathogens have made
relatively little impact in diagnostic microbiology laboratories
due to the common decision to use expensive commercially produced
tests rather than the cheaper alternative of developing one's own
tests or introducing tests developed by other workers. PCR
Detection of Microbial Pathogens, Second Edition presents
alternatives to commercially produced PCR methods to detect
microbial pathogens. Although most of the chapters in this book are
devoted to the detection of specific pathogens, the first chapters
in this book should appeal to anyone working in this field
regardless of their particular interests. Although PCR tests can
often be made to work with relatively little effort, it is often
unclear how efficient the PCR test is, how inhibitory the specimen
containing the pathogen of interest is and how the test can be
quality controlled. All of which are of great importance in
developing tests for diagnostic use. These topics are covered in
great depth at the beginning of the book. The main part of the book
is devoted to describing methods for the detection of a wide range
of pathogens and from widely different specimens and situations.
Written in the highly successful Methods in Molecular Biology (TM)
series format, chapters contain introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls. Authoritative and
accessible, PCR Detection of Microbial Pathogens, Second Edition
serves microbiologists regardless of their particular interest
because, when used together with the general principles, the sheer
variety of procedures provided here enables the reader to design
and introduce diagnostic tests in the laboratory with confidence.
The 18th century was a wealth of knowledge, exploration and rapidly
growing technology and expanding record-keeping made possible by
advances in the printing press. In its determination to preserve
the century of revolution, Gale initiated a revolution of its own:
digitization of epic proportions to preserve these invaluable works
in the largest archive of its kind. Now for the first time these
high-quality digital copies of original 18th century manuscripts
are available in print, making them highly accessible to libraries,
undergraduate students, and independent scholars.The Age of
Enlightenment profoundly enriched religious and philosophical
understanding and continues to influence present-day thinking.
Works collected here include masterpieces by David Hume, Immanuel
Kant, and Jean-Jacques Rousseau, as well as religious sermons and
moral debates on the issues of the day, such as the slave trade.
The Age of Reason saw conflict between Protestantism and
Catholicism transformed into one between faith and logic -- a
debate that continues in the twenty-first century.++++The below
data was compiled from various identification fields in the
bibliographic record of this title. This data is provided as an
additional tool in helping to insure edition identification:
++++British LibraryT064802With a half-title. Norwich?]: Printed for
the author and may be had of the booksellers in general, 1791.
4],77, 1]p.; 8
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