Unlike some other reproductions of classic texts (1) We have not used OCR(Optical Character Recognition), as this leads to bad quality books with introduced typos. (2) In books where there are images such as portraits, maps, sketches etc We have endeavoured to keep the quality of these images, so they represent accurately the original artefact. Although occasionally there may be certain imperfections with these old texts, we feel they deserve to be made available for future generations to enjoy.

PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one's own tests or introducing tests developed by other workers. PCR Detection of Microbial Pathogens, Second Edition presents alternatives to commercially produced PCR methods to detect microbial pathogens. Although most of the chapters in this book are devoted to the detection of specific pathogens, the first chapters in this book should appeal to anyone working in this field regardless of their particular interests. Although PCR tests can often be made to work with relatively little effort, it is often unclear how efficient the PCR test is, how inhibitory the specimen containing the pathogen of interest is and how the test can be quality controlled. All of which are of great importance in developing tests for diagnostic use. These topics are covered in great depth at the beginning of the book. The main part of the book is devoted to describing methods for the detection of a wide range of pathogens and from widely different specimens and situations. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, PCR Detection of Microbial Pathogens, Second Edition serves microbiologists regardless of their particular interest because, when used together with the general principles, the sheer variety of procedures provided here enables the reader to design and introduce diagnostic tests in the laboratory with confidence.

PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one's own tests or introducing tests developed by other workers. PCR Detection of Microbial Pathogens, Second Edition presents alternatives to commercially produced PCR methods to detect microbial pathogens. Although most of the chapters in this book are devoted to the detection of specific pathogens, the first chapters in this book should appeal to anyone working in this field regardless of their particular interests. Although PCR tests can often be made to work with relatively little effort, it is often unclear how efficient the PCR test is, how inhibitory the specimen containing the pathogen of interest is and how the test can be quality controlled. All of which are of great importance in developing tests for diagnostic use. These topics are covered in great depth at the beginning of the book. The main part of the book is devoted to describing methods for the detection of a wide range of pathogens and from widely different specimens and situations. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, PCR Detection of Microbial Pathogens, Second Edition serves microbiologists regardless of their particular interest because, when used together with the general principles, the sheer variety of procedures provided here enables the reader to design and introduce diagnostic tests in the laboratory with confidence.

Classically educated, Mark Wilks (c.1760-1831) joined the Madras army of the East India Company and would go on to serve as political resident at the court of Mysore from 1803 to 1808. He also later served as governor of Saint Helena from 1813 to 1816, during which time he witnessed the arrival of Napoleon Bonaparte. Elected to the Manx parliament in 1816, Wilks became its speaker in 1826 and was elected a fellow of the Royal Society in the same year. Drawing on state records and his own experiences, he first published this acclaimed three-volume history of the kingdom of Mysore between 1810 and 1817. Volume 1, an 1820 reprint of the 1810 original, covers the early Hindu dynasties, the Mughal period, the emergence of a separate Mysore under Hyder Ali (c.1720-82), and early French and British dealings in southern India to 1767.

Classically educated, Mark Wilks (c.1760-1831) joined the Madras army of the East India Company and would go on to serve as political resident at the court of Mysore from 1803 to 1808. He also later served as governor of Saint Helena from 1813 to 1816, during which time he witnessed the arrival of Napoleon Bonaparte. Elected to the Manx parliament in 1816, Wilks became its speaker in 1826 and was elected a fellow of the Royal Society in the same year. Drawing on state records and his own experiences, he first published this acclaimed three-volume history of the kingdom of Mysore between 1810 and 1817. Volume 2 details the first (1767-9) and second (1779-84) Anglo-Mysore wars, concluding with the death of Hyder Ali (c.1720-82) and the accession of Tipu Sultan (1750-99).

Classically educated, Mark Wilks (c.1760-1831) joined the Madras army of the East India Company and would go on to serve as political resident at the court of Mysore from 1803 to 1808. He also later served as governor of Saint Helena from 1813 to 1816, during which time he witnessed the arrival of Napoleon Bonaparte. Elected to the Manx parliament in 1816, Wilks became its speaker in 1826 and was elected a fellow of the Royal Society in the same year. Drawing on state records and his own experiences, he first published this acclaimed three-volume history of the kingdom of Mysore between 1810 and 1817. Volume 3 narrates the history of the third (1789-92) and fourth (1798-9) Anglo-Mysore wars, the capture of Seringapatam and the death of Tipu Sultan (1750-99).