This manual presents a comprehensive guide to the most up-to-date technologies and applications as well as providing an overview of the theory of this increasingly important technique. It also discusses a wide range of RT-PCR applications including clinical diagnostics, biodefence, RNA expression studies, and more.

Few events have had a more profound impact on the social and cultural upheavals of the Sixties than the psychedelic revolution spawned by the spread of LSD. This book for the first time tells the full and astounding story--part of it hidden till now in secret Government files--of the role the mind-altering drug played in our recent turbulent history and the continuing influence it has on our time.
And what a story it is, beginning with LSD's discovery in 1943 as the most potent drug known to science until it spilled into public view some twenty years later to set the stage for one of the great ideological wars of the decade. In the intervening years the CIA had launched a massive covert research program in the hope that LSD would serve as an espionage weapon, psychiatric pioneers came to believe that acid would shed light on the perplexing problems of mental illness, and a new generation of writers and artists had given birth to the LSD sub-culture.
Acid Dreams is a complete social history of the psychedelic counter-culture that burst into full view in the Sixties. With new information obtained through the Freedom of Information Act, the authors reveal how the CIA became obsessed with LSD during the Cold War, fearing the Soviets had designs on it as well. What follows is one of the more bizarre episodes in the covert history of U.S. intelligence as the search for a "truth drug" began to resemble a James Bond scenario in which agents spied on drug-addicted prostitutes through two-way mirrors and countless unwitting citizens received acid with sometimes tragic results.
The story took a new turn when Captain Al Hubbard, the first of a series of "Johnny Appleseeds" of acid, began to turn on thousands of scientists, businessmen, church figures, policemen, and others from different walks of life.
Timothy Leary, Ken Kesey and his band of Merry Pranksters, Allen Ginsberg and the Beat generation, the Diggers and the Age of Golden Anarchy in Haight-Ashbury, William Mellon Hitchcock, Abbie Hoffman and the Yippies, the Beatles--these are just some of a motley cast of characters who stride through the pages of this compelling chronicle. What impact did the widespread use of LSD have on the anti-war movement of the late Sixties? Acid Dreams traces the way the drug intensified each stage of counter-cultural transition to break the "mind-forged manacles" of a new generation in rebellion.
In Acid Dreams, Martin Lee and Bruce Shalin have written the history of a time still only dimly understood. The events they recount and the facts they uncover supply an important missing piece of the puzzle of a crucial decade in our recent past.

Praise
"Engaging throughout. . . . At once entertaining and disturbing."--Andrew Weil, M.D., The Nation
"Marvelously detailed . . . loaded with startling revelations."--Los Angeles Daily News
"Excellent. . . . Captivating. . . . A generalist's history that should replace all others."--San Francisco Chronicle
"A landmark contribution to the sociopolitical history of the U.S. . . . Some of the liveliest, most absorbing, best-documented historical analyses to appear in recent years. . . . A seminal contribution to understanding America's most turbulent modern decade."--Choice
"This funny and irreverent book brings it all back."--The Washington Post
"Recounts some of the most bizarre incidents in the history of U.S. intelligence."--The Boston Globe
"A monumental social history of psychedelia."--The Village Voice
"A blistering expose of CIA drug experimentation on Americans. It's all there."--John Stockwell
"Highly readable. . . . Well researched. . . . Filled with entertaining and bizarre episodes."--The Detroit Free Press
"An important study of cultural history. . . . The scholarship is exquisite and the methods sensible."--Allen Ginsberg
"An engrossing account of a period . . . when a tiny psychoactive molecule affected almost every aspect of Western life."--William S. Burroughs
"A missing link, a work of combat history, a devastating combination of facts and poetry that is bound to arouse controversy."--Paul Krassner
"An important historical synthesis of the spread and effects of a drug that served as a central metaphor for an era."--John Sayles

This is the great American pot story, a dramatic social exploration of a plant that sits at the nexus of political, legal, medical, and scientific discourse. From its ancient origins, to its cutting-edge therapeutic benefits, to its role in a culture war that has never ceased, marijuana has evolved beyond its own illicit subculture into a dynamic, multibillion-dollar industry. Since 1996, when California voters approved Proposition 215, dozens of state and local governments across the country have circumvented federal authority to legalize marijuana for medical purposes. Mining the plant's rich botanical properties, medical researchers are now develop-ing promising marijuana-based treatments for cancer, heart disease, Alzheimer's, diabetes, chronic pain, and many other conditions. Martin Lee, an award-winning investigative journalist, examines this complex landscape where legal ambiguity meets scientific breakthrough in a panoramic, character-driven saga.

"Committed, eloquent writings that plumb teh psychological and political complexities of mass-mediated experience." --San Francisco Chronicle

"An essential text." --Utne Reader

"More than helping to detect bias, "Unreliable Sources" tells the stories behind the stories called news. It should help build a national constituency for liberating media from all major constraints-- corporate as well as governmental." --George Gerbner, Dean Emeritus and Professor of Communications, The Annenberg School for Communications

"You gotta love these guys. Not only have Lee and Solomon written a timely consumer primer on conservative bias in reporting, they've done it with humor." --Washington Journalism Review

A vital handbook for deciphering widespread media bias. "Unreliable Sources" dissects news coverage of a wide range of issues-- taxes, the Persian Gulf, social security, abortion, drugs, environmental pollution, U.S.-Soviet relations, terrorism, the Third World-- and exposes the key stories that have been censored or glossed over by major media.