This book constitutes the refereed proceedings of the 8th IAPR International Conference on Pattern Recognition in Bioinformatics, PRIB 2014, held in Stockholm, Sweden in August 2014. The 9 revised full papers and 9 revised short papers presented were carefully reviewed and selected from 29 submissions. The focus of the conference was on the latest Research in Pattern Recognition and Computational Intelligence-Based Techniques Applied to Problems in Bioinformatics and Computational Biology.