This up-to-date book compiles the basic and advanced laboratory techniques of mammalian cell culture. It is divided into four major sections encompassing the basics of cell culture, nucleic acid and protein isolation, cell-staining techniques, and cell transfection and single-cell analysis. The topics include aseptic handling, media preparation, and passaging of cells. The book also outlines downstream assays such as nucleic acid and protein isolation from in-vitro cell cultures. It provides elaborate stepwise protocols, key points, and troubleshooting tips. Key Features Provides step-wise protocols, flowcharts, key points to consider and troubleshooting tips Covers cellular staining using fluorescent dyes, genetic manipulation of cells via transfection, and an introduction to single-cell analyses Discusses basics in cell culture and downstream applications including gene and protein expression analysis Includes the principles underlying each of the techniques and provides a detailed methodology to practice Explores the whole range of techniques- from basic to downstream applications and advanced methods The book is essential for students and researchers in the field of life sciences, biotechnology, genetics, and molecular biology.

Fiber-reinforced polymer composites exhibit better damping characteristics than conventional metals due to the viscoelastic nature of the polymers. There has been a growing interest among research communities and industries in the use of natural fibers as reinforcements in structural and semi-structural applications, given their environmental advantages. Knowledge of the vibration and damping behavior of biocomposites is essential for engineers and scientists who work in the field of composite materials. Vibration and Damping Behavior of Biocomposites brings together the latest research developments in vibration and viscoelastic behavior of composites filled with different natural fibers. Features: Reviews the effect of various types of reinforcements on free vibration behavior Emphasizes aging effects, influence of compatibilizers, and hybrid fiber reinforcement Explores the influence of resin type on viscoelastic properties Covers the use of computational modeling to analyze dynamic behavior and viscoelastic properties Discusses viscoelastic damping characterization through dynamic mechanical analysis. This compilation will greatly benefit academics, researchers, advanced students, and practicing engineers in materials and mechanical engineering and related fields who work with biocomposites. Editors Dr. Senthil Muthu Kumar Thiagamani, Kalasalinagam Academy of Research and Education (KARE), India Dr. Md Enamul Hoque, Military Institute of Science and Technology (MIST), Bangladesh Dr. Senthilkumar Krishnasamy, King Mongkut's University of Technology North Bangkok KMUTNB, Thailand Dr. Chandrasekar Muthukumar, Hindustan Institute of Technology & Science (HITS), India Dr. Suchart Siengchin, King Mongkut's University of Technology North Bangkok KMUTNB, Thailand

Green Biocomposites for Biomedical Engineering: Design, Properties, and Applications combines emergent research outcomes with fundamental theoretical concepts relevant to processing, properties and applications of advanced green composites in the field of biomedical engineering. The book outlines the design elements and characterization of biocomposites, highlighting each class of biocomposite separately. A broad range of biomedical applications for biocomposites is then covered, with a final section discussing the ethics and safety regulations associated with manufacturing and the use of biocomposites. With contributions from eminent editors and recognized authors around the world, this book is a vital reference for researchers in biomedical engineering, materials science and environmental science, both in industry and academia.

Metal-Organic Frameworks for Chemical Reactions: From Organic Transformations to Energy Applications brings together the latest information on MOFs materials, covering recent technology in the field of manufacturing and design. The book covers different aspects of reactions from energy storage and catalysts, including preparation, design and characterization techniques of MOFs material and applications. This comprehensive resource is ideal for researchers and advanced students studying metal-organic frameworks in academia and industry. Metal-organic frameworks (MOFs) are nanoporous polymers made up of inorganic metal focuses connected by natural ligands. These entities have become a hot area of research because of their exceptional physical and chemical properties that make them useful in di?erent ?elds, including medicine, energy and the environment. Since combination conditions strongly a?ect the properties of these compounds, it is especially important to choose an appropriate synthetic technique that produces a product with homogenous morphology, small size dispersion, and high thermal stability.

Advanced Polymer Nanocomposites: Science Technology and Applications presents a detailed review of new and emerging research outcomes from fundamental concepts that are relevant to science, technology and advanced applications. Sections cover key drivers such as the rising demand for lightweight and high strength automotive parts, the need for sustainable packaging materials and conservation of flavor in the food, drinks and beverages industries, and defense initiatives such as ballistic protection, fire retardation and electromagnetic shielding. With contributions from international authors working at the cutting-edge of research, this book will be an essential reference resource for materials scientists, chemists, manufacturers and polymer engineers. Through recent advances in nanotechnology, researchers can now manipulate atoms to create materials and products that are changing the way we live our lives. These materials have enhanced properties, such as tensile strength, impact and scratch resistance, electrical and thermal conductivity, thermal stability and fire resistance.