Efficacies of locally isolated bacterium, Bacillus sphaericus total proteins have been investigated against the 3rd instar larvae of wild mosquito Culex quinquefasciatus under the laboratory conditions.The LC50 values were recorded as 19.069 ug/ul at 72 h posttreatment. Association of both the proteins of 42 kDa and 51 kDa were found to require for mosquitocidal efficacy. The thermal stability properties of the Bin proteins were performed at various temperature ranges from 40 C, 50 C, 60 C, 70 C, 80 C & 90 C. The efficacy of the heat incubated Bin proteins were tested and showed that the binary proteins were active up to 70 C but lost their activity when treated at 80 C and 90 C. Among the two Bin proteins, 42 kDa proteins were found to be more stable than the 51 kDa protein. This data suggest that both the proteins were required for mosquitocidal efficacy for the production of bioinsectiside which would be used as a biological control of mosquito."

The flour beetle T.castaneum is an important worldwide pest of stored products (Mabbet, 1993) that feeding upon a wide range of commodities, especially wheat flour. Nutrition involves the nutrient materials, which are available in foodstuff and the physiological, and biochemical processes by which these nutrient materials are transformed into body tissues and energy for all the activities as life demands.Variation in the quantity and quality of an acceptable diet can have profound effect on insect development. The quantities of protein and amino acids ingested are important for optimal growth and development. The ultimate size of the insects, reflected in the gain in body nitrogen increase as nitrogen intake is increased (Hone and Watanabe, 1983). Commonly as food intake decrease, the duration of development is extended and the insect becomes smaller and lighter in weight.