Experimental Analysis of Enzyme Mechanism Using Isotope Effects, Volume 596, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this comprehensive update include Measurement of enzyme binding isotope effects, Chemical ligation and isotope labeling to locate dynamic effects, Measurement of heavy enzyme isotope effects, Extracting kinetic isotope effects from a global analysis of reaction progress curves, KIE of metabolic flux and enzymes, Solvent and Primary KIE on Flavin Enzymes, and The Rapid Determination of Primary Deuterium Isotope Effects on Enzyme-Catalyzed Proton Transfer at Carbon in 50/50 HOH/DOD. Readers who are interested in applying or understanding this research will find useful methods currently used for measuring isotope effects on solution and enzyme reactions.

Special Operations Forces (SOF) are elite military units with special training and equipment that can infiltrate into hostile territory through land, sea or air to conduct a variety of operations, many of them classified. SOF personnel undergo rigorous selection and lengthy specialised training. The U.S. Special Operations Command (USSOCOM) oversees the training, doctrine and equipping of all U.S. SOF units. This book examines the background and issues for Congress of the U.S. Special Operations Forces with a focus on their history, mission and priorities, as well as their core activities.