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Enzymology at the Membrane Interface, the latest volume in the
Methods in Enzymology series, covers a subset of enzymes that work
in the environment of the biological cell membrane. This field,
called interfacial enzymology, involves a special series of
experimental approaches for the isolation and study of these
enzymes.
It is hard to think of a protein in eukaryotic cells that does not
undergo some type of posttranslational modification. The covalent
attachment of l- ids to proteins, protein lipidation, occurs for a
few thousand proteins. Several functions for protein lipidation are
known. Protein lipids may target proteins to specific cellular
membranes, they may serve as molecular switches that allow
cytosol-to-membrane transfer, they may direct protein-protein
compl- ation, and they may stabilize protein structure. In cases
such as the fatty a- lation of intracellular loops of transmembrane
proteins, the funtions of the protein lipidations are not known.
This volume-Protein Lipidation Protocols-provides detailed meth-
ologies for the study of these processes. Since this is a rapidly
growing field, many new experimental techniques have been
developing over the past few years. All of the experimental
techniques described in this volume have emerged during this time.
The editor has made a special effort to include only those
techniques that have not been previously described in a "hands-on"
f- mat.
It is hard to think of a protein in eukaryotic cells that does not
undergo some type of posttranslational modification. The covalent
attachment of l- ids to proteins, protein lipidation, occurs for a
few thousand proteins. Several functions for protein lipidation are
known. Protein lipids may target proteins to specific cellular
membranes, they may serve as molecular switches that allow
cytosol-to-membrane transfer, they may direct protein-protein
compl- ation, and they may stabilize protein structure. In cases
such as the fatty a- lation of intracellular loops of transmembrane
proteins, the funtions of the protein lipidations are not known.
This volume-Protein Lipidation Protocols-provides detailed meth-
ologies for the study of these processes. Since this is a rapidly
growing field, many new experimental techniques have been
developing over the past few years. All of the experimental
techniques described in this volume have emerged during this time.
The editor has made a special effort to include only those
techniques that have not been previously described in a "hands-on"
f- mat.
Enzymology at the Membrane Interface: Intramembrane Proteases,
Volume 584, the latest release in the Methods in Enzymology series,
covers a subset of enzymes that work in the environment of the
biological cell membrane. This field, called interfacial
enzymology, involves a special series of experimental approaches
for the isolation and study of these enzymes.
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