This second edition details new and updated methods on different antibody libraries, along with novel approaches for antibody discovery. Chapters focuses on the construction of antibody libraries, antibody expression, complementary approaches for antibody selection, and other phage display related applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Phage Display: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.   

This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field of antibody phage display. This volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject.

Die vollstandig uberarbeitete zweite Auflage bietet eine umfassende Einfuhrung in die grundlegenden Technologien wie auch in die vielfaltigen Anwendungsgebiete rekombinant hergestellter und verbesserter Antikoerper und ihrer unzahligen Varianten. Die einzelnen Kapitel gehen detailliert auf die Herstellung, die Charakterisierung, die Produktion und die Anwendungsgebiete fur rekombinante Antikoerper in Forschung, Diagnostik und Therapie ein. Die LeserInnen werden schrittweise an die zugrunde liegenden Prinzipien herangefuhrt und erhalten so einen leichten Zugang zu den komplexen Themen. Zahlreiche anschauliche Abbildungen, die einem ausgereiften didaktischen Farb- und Formenschema folgen, helfen dabei, die Zusammenhange nachzuvollziehen. So koennen sich auch LeserInnen mit wenigen Vorkenntnissen die komplexe Welt der rekombinanten Antikoerper in der Tiefe erschliessen.