The critically acclaimed laboratory standard for more than forty years, Methods in Enzymologyis one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
This volume and its companions (Volumes 330 and 334) cover all current knowledge concerning hyperthermophilic enzymes. Major topics in this volume include the enzymes of central metabolism and respiratory enzymes.

General Description of the Volume:
This is the Cumulative Subject Index for Methods in Enzymology Volumes 290-319. It will supplement the Methods in Enzymology Index Volumes 75, 95, 120, 140, 175, 199,299, 265, and 285. In addition to the Subject Index, this volume includes the complete Contents of each volume indexed, and a Contributor's Index.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Volume 324 of Methods in Enzymology supplements Volume 166. It includes genetic information (cloning, gene expression) and information on human genetic diseases not available when Volume 166 was published.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Preparation of substrates and assay of enzymes
* Cloning, expression, and purification of enzymes
* Detection and consequences of genetic defects
* Regulation and expression of enzymes

General Description of the Volume:
The articles in this Methods in Enzymology volume reflect the integration of molecular genetics with photosynthesis. They focus on the genetic engineering of the photosynthesis apparatus, new mechanisms for genetic selection of photosynthetic mutants, biophysical and biochemical analysis of Photosystem I and Photosystem II Mutations, and Photosynthetic Gene Expression.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Genetic engineering of the photosynthesis apparatus
* New mechanisms for genetic selection of photosynthetic mutants
* Biophysical and biochemical analysis of Photosystem I and Photosystem II Mutations
* Photosynthetic Gene Expression

General Description of the Volume:
This Methods in Enzymology volume continues the description (initiated in Volume 85) of the purification and assay of this diverse group of proteins involved in cytoplasmic organization. It discusses some of the more promising attempts to apply techniques of molecular biology genetics and molecular cytochemistry to these proteins.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Major topics covered are:
* Analysis of Actinomyosin-Related Systems and of Microtubule-Related Systems
* Other Cytoskeletal Systems
* Cell-Free and Genetic Systems
* Force Production Assays
* General Methods

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 285 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Catalysts of Protein Folding: Protein Disulfide Isomerases, Cis-trans Peptidyl Prolyl Isomerases
* Accessory Proteins: Chaperonins, Cochaperonins, Pap Proteins, Sec Proteins
* Physical methods for investigation of interactions between chaperones and their substances
* Cotranslational protein folding, cell-free protein synthesis and associated methods

This cumulative subject index for Methods in Enzymology covers volumes 263, 264, and 266-289. In addition to the Subject Index, this volume includes the complete contents of each volume indexed and a Contributor's Index.

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* This volume and its companion Volumes 279, 280, and 281 provide
* A collation of the most recent and useful methods for the identification, preparation, and quantification of vitamins and coenzymes
* Details on physical, chemical, and biological properties of vitamins and coenzymes
* Chemical and biological syntheses of vitamins, coenzymes, and their analogs
* Aspects of transport and metabolism of vitamins and coenzymes

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Fluorescence in biophysics
* Fluorescence as a tool for studying proteins
* Determination of ground-state dissociation constant by fluorescence spectroscopy
* Fluorescence spectroscopy of protein-protein and protein-nucleic acid interactions
* Fluorescence methods for studying equilibrium macromolecule-liquid interactions
* Methods for detecting molecular clusters on cell surfaces

Nitric oxide, recently named"Molecule of the Year"by the journal "Science," impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organismal biology, as well as in fundamental chemistry. This volume and its companion Volume 269 highlight the most current and up-to-date methods used to research the physiology and toxicity of nitric oxide in cellular and organismal systems. It will be a valuable resourcefor the experienced researcher as well as for those newly entering the field.
Key Features
* Generation, detection, and characterization of biological and chemical sources of nitric oxide
* Biochemistry and molecular biology of enzymes and proteins associated with nitric oxide metabolism

General Description of the Volume:
The role of low molecular weight cofactors (often vitamins) in enzyme catalysis has been discussed in many earlier volumes of Methods in Enzymology. Exciting new results indicate that redox-active prosthetic groups can also arise from pre-existing amino acid side chains in proteins. In this volume, methods are described for the detection and characterization of such prosthetic groups in a range of enzyme systems.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Among the redox-active prosthetic groups covered are:
* Topa quinones
* Pyrroloquinoline quinones
* Quinoproteins
* Tryptophan tryptophylquinones

General Description of the Volume:
Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport. This volume plus its companions Volumes 256 and 257 cover all the biochemical and biological assays currently in use for analyzing the role of small GTPases in many aspects of cell biology at the molecular level.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Expression, purification, and posttranslational modification
* Guanine nucleotide exchange and hydrolysis
* Cell expression and analysis "in Vitro"
* Biological activity

The Cumulative Subject Index for Volumes 195(198 and 200(227 includes the contents of the volumes covered, the subject index, contributor index, and a complete list of all the volumes published in the series. This volume supplements the Methods in Enzymology Cumulative Index Volumes 33, 75, 95, 120, 140, 175, and 199.

The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Prevention of oxidative stress
* Redox regulation of metabolism
* Toxicology
* Carcinogenesis

General Description of the Volume:
This volume, as do the other "Enzyme Kinetics and Mechanism" volumes in the Methods in Enzymology series, provides treatment of dynamic and chemical approaches for investigating enzyme catalysis and regulation, as well as designing metabolic inhibitors. It will greatly interest those involved in enzyme chemistry, metabolic control, and drug design. It should also interest those developing commercial applications for enzymes whose properties have been re-engineered using recombinant DNA technology and site-directed mutagenesis.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Mechanisms of enzyme catalysis and inhibition
* Enzyme structure and function
* Regulatory control of enzymes
* Action of catalytic antibodies and ribozymes

Neoglycoconjugates are not only useful for the basic understanding of protein-carbohydrate interactions, but they have many practical applications as well. They are powerful reagents in many cell biology studies and excellent tools for the isolation and characterization of animal and plant lectins, separation of cells, as well as for the targeting of drugs, artificial vaccines, and diagnostic reagents. Volume 247 and its companion Volume 242 contain many practical methods on how to prepare and use neoglycoconjugates. Volume 242 deals with synthesis and 247 with biomedical applications.

Neoglycoconjugates are not only useful for the basic understanding of protein-carbohydrate interactions, but they have many practical applications as well. They are powerful reagents in many cell biology studies and excellent tools for the isolation and characterization of animal and plant lectins, separation of cells, as well as for the targeting of drugs, artificial vaccines, and diagnostic reagents. Volume 247 and its companion Volume 242 contain many practical methods on how to prepare and use neoglycoconjugates. Volume 242 deals with synthesis and 247 with biomedical applications.

This volume presents methods related to the role of anaerobic dissimilatory cycles and sulfur metabolism.
Key Features
@bul* Sulfate Transport
* Dissimilatory Sulfate Metabolism
* Dehydrogenases
* Hydrogenases from sulfate-reducing bacteria
* Electron carrier protein sulfate-reducing bacteria
* Terminal reductases of sulfate-reducing bacteria molecular biology
* Dissimilatory sulfur reduction
* Oxidation of reduction compounds
* Metabolism of polythinates
* Special techniques

Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 233 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease. It includes techniques and assays of the activity of biothiols in soluble systems, their interaction with lipids and proteins, and the various new thiol enzymes, such as thioltransferases, being elucidated. Antioxidant reactions and gene regulation are also included.

Hemoglobin has been involved in the most significant advances in our understanding of modern genetics and molecular biology. Now, hemoblobin is again central to a new area: development of artificial blood (blood substitute.) This volume of Methods in Enzymology and its companion, Volume 232, will be indispensable to anyone with a serious interest in this emerging field. They completely update and extend the information presented in Volume 76 published a decade ago.
Key Features
* Preparation, purification, and analysis of human hemoglobin and of various non-human hemoglobins
* Preparation of hemoglobin hybrids and of model heme compounds
* Preparation of chemically modified hemoglobins and of recombinant hemoglobins
* Analytical procedures presented for studying hemoglobin stability and degradation and for the formation of xenobiotic adducts of hemoglobin

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

This volume and its companion, volume 214 of Methods in Enzymology, present a comprehensive, state-of-the-art compilation of the molecular and cellular methodology needed for pursuing research with carotenoids.

To understand the structures and dynamics of DNA and its interactions with ligands, a complete analysis of its confirmational flexibility is required. This volume and its companion, Volume 212 of Methods in Enzymology, collate in one source all the methods and approaches needed to achieve this goal.

The aim of this volume is to brief researchers of the importance of data analysis in enzymology, of the modern methods that have developed concomitantly with computer hardware, and of the need to validate their computer programs with real and synthetic data to ascertain that the results produced are what they expected.

This volume provides a compilation of recent methods for studying protein phosphorylation.