Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF- B activation, regulation, and function. NF-kappa B: Methods and Protocols guides readers through standard approaches to detect NF- B pathway activation, detection and analysis of NF- B signaling, and methods to study the control of NF- B signaling. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, NF-Kappa B: Methods and Protocols provides a timely and invaluable resource for researchers seeking to perform experiments aimed at understanding the role of NF- B signaling in health and disease.