|
Showing 1 - 5 of
5 matches in All Departments
Trophoblast cells coordinate the activities of maternal and
embryonic tissues by secreting hormones, cytokines, and various
growth factors that selectively and specifically gain access to
maternal and embryonic compartments. Abnormalities associated with
trophoblast cell growth, differentiation, or function result in
impaired embryonic development. Understanding the complexities of
the trophoblast cell signaling system was the focus of the Serono
Symposia, USA conference entitled Tropho blast Cells: Pathways for
Maternal-Embryonic Communication, held August 6-9, 1992, in Las
Vegas, Nevada. The conference was designed to provide a forum for
morphologists, cell biologists, endocrinologists, and molecular
biologists and for scientists investigating primate, ru minant, and
rodent trophoblast biology. An important outcome of the conference
was the communication achieved between basic scientists and
clinicians. This volume represents the contributions of the invited
symposium speakers. The opening keynote address of the conference
was entitled "Chorio carcinoma and the Embryo" and was presented by
G. Barry Pierce, M. D., Centennial Distinguished Research Professor
of the University of Colorado School of Medicine. The address is
not represented in this volume, but deserves a special comment. Dr.
Pierce's scientific con tributions have significantly influenced
our understanding of trophoblast cells. Dr. Pierce, together with
Dr. A. Rees Midgley, identified the origin of syncytial trophoblast
cells of the primate placenta and discovered that these cells were
responsible for the production of chorionic gonadotropin (J Exp Med
1962;115:289-94; Am J Pathol 1963;43:929-43; and Science
1963;141:349-50)."
A collection of cutting-edge laboratory techniques for the study of
trophoblast and placental biology. The techniques presented range
from experimental animal models, to animal and human placental
organ and cell culture systems, to morphological, biochemical, and
molecular strategies for assessing trophoblast/placental growth,
differentiation and function. Volume 1 provides readily
reproducible protocols for studying embryo-uterine implantation,
trophoblast cell development, and the organization and molecular
characterization of the placenta. Highlights include strategies for
the isolation and culture of trophoblast cells from primates,
ruminants, and rodents, and precise guidance to the molecular and
cellular analysis of the placental phenotype. A companion second
volume concentrates on methods for investigating placental
function.
A collection of cutting-edge laboratory techniques for the study of
trophoblast and placental biology. The techniques presented range
from experimental animal models, to animal and human placental
organ and cell culture systems, to morphological, biochemical, and
molecular strategies for assessing trophoblast/placental growth,
differentiation and function. Volume 1 provides readily
reproducible protocols for studying embryo-uterine implantation,
trophoblast cell development, and the organization and molecular
characterization of the placenta. Highlights include strategies for
the isolation and culture of trophoblast cells from primates,
ruminants, and rodents, and precise guidance to the molecular and
cellular analysis of the placental phenotype. A companion second
volume concentrates on methods for investigating placental
function.
The aim of the two-volume set of Placenta and Trophoblast: Methods
and Protocols is to offer contemporary approaches for studying the
biology of the placenta. The chapters contained herein also address
critical features of the female organ within which the embryo is
housed, the uterus, and some aspects of the embryo-fetus itself,
particularly those of common experimental animal models. In keeping
with the organization used effectively in other volumes in this
series, each chapter has a brief introduction followed by a list of
required items, protocols, and notes designed to help the reader
perform the expe- ments without difficulty. In both volumes,
sources of supplies are given and illustrations highlight
particular techniques as well as expected outcomes. A key aspect of
these volumes is that the contributors are at the forefronts of
their disciplines, thus ensuring the accuracy and usefulness of the
chapters. Placenta research has progressed rapidly over the past
several decades by taking advantage of the technical advances made
in other fields. For example, the reader will note that many
techniques, such as reverse transcriptase po- merase chain
reaction, northern and western blotting, microarray analyses and in
situ hybridization experiments, are routinely used for dissecting a
wide range of experimental questions. Protein analysis and
functional experiments on tissues and cells that comprise the
maternal-fetal interface benefit from studies in en- crinology,
immunology, and developmental biology. These volumes also present
new ideas on investigating gene imprinting and gene transfer via
viral vectors.
The aim of the two-volume set of Placenta and Trophoblast: Methods
and Protocols is to offer contemporary approaches for studying the
biology of the placenta. The chapters contained herein also address
critical features of the female organ within which the embryo is
housed, the uterus, and some aspects of the embryo-fetus itself,
particularly those of common experimental animal models. In keeping
with the organization used effectively in other volumes in this
series, each chapter has a brief introduction followed by a list of
required items, protocols, and notes designed to help the reader
perform the expe- ments without difficulty. In both volumes,
sources of supplies are given and illustrations highlight
particular techniques as well as expected outcomes. A key aspect of
these volumes is that the contributors are at the forefronts of
their disciplines, thus ensuring the accuracy and usefulness of the
chapters. Placenta research has progressed rapidly over the past
several decades by taking advantage of the technical advances made
in other fields. For example, the reader will note that many
techniques, such as reverse transcriptase po- merase chain
reaction, northern and western blotting, microarray analyses and in
situ hybridization experiments, are routinely used for dissecting a
wide range of experimental questions. Protein analysis and
functional experiments on tissues and cells that comprise the
maternal-fetal interface benefit from studies in en- crinology,
immunology, and developmental biology. These volumes also present
new ideas on investigating gene imprinting and gene transfer via
viral vectors.
|
|