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Forensic and Clinical Applications of Solid Phase Extraction presents a compendium of methods and supporting data that allows the scientist to perform procedures using solid phase extraction to isolate the compounds of interest in the field of clinical and forensic toxicology. The more experienced researchers may vary the presented methods to achieve specific extractions. The methods presented have been used for hundreds of different compounds, thus the technique offers comprehensive technology to the researcher. The book teaches the required fundamentals. The methodologies make use of the latest developments in copolymeric bonded phases. By presenting organized and easy to use methodologies, this volume will encourage a wider acceptance of the technology and help many researchers to solve their individual separation problems. This volume will be of interest to clinical chemists, toxicologists, medical examiners, criminologists, hospital technicians, pharmaceutical and environmental chemists and medical school technicians.
This complete laboratory reference manual explains the principles behind solid phase extraction (SPE) and provides readily reproducible protocols for solving extraction problems in forensic and clinical chemistry. Numerous actual chromatograms, based on original research and diverse applications, demonstrate the technique and the results that can be achieved. Extensive appendices allow fast access to frequently needed information on reagents, the preparation of solutions and buffers, milliequivalent and millimode calculations, buffers and pKa for SPE, and a complete RapidTrace (R) technical manual. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and on avoiding known pitfalls.
This second edition continues to present a compendium of methods and supporting data that allows the scientist to perform cookbook methods using solid phase extraction to isolate compounds. The methods presented have been used for hundreds of different compounds, thus the technique offers comprehensive technology to the researcher. The major changes will be in expanded methods (new and contemporary drugs), a new section on hardware, new techniques which have evolved, a reference expansion on pKa data and a compilation of helpful web links.
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