Reflecting developments in genome editing, this third edition volume fully updates a collection of key techniques for the study of functional genomics. The book is broken up into sections on bioinformatics, DNA, RNA, and protein analysis, as well as a closing section entitled "From Genotype to Phenotype." Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Updated and authoritative, Functional Genomics: Methods and Protocols, Third Edition seeks to aid scientists in establishing or extending technologies and techniques in their laboratories.

Over the last decade Life Science has undergone an accelerated evolution, culminating in the -omics era characterized by the development of a multitude of high throughput methods that are becoming more routinely applied in biochemistry labs. In Functional Genomics: Methods and Protocols, Second Edition expert researchers in the field detail many of the methods which are now commonly used for studies in the life sciences focusing on the dynamic aspects of the transcriptome, proteome and metabolome, respectively.Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Functional Genomics: Methods and Protocols, Second Edition seeks to aid scientists in establishing or extending technologies and techniques in their laboratories.

Die Autoren führen in relationale (SQL) und nicht-relationale (NoSQL) Datenbanken ein. Die 9. Auflage erklärt die drei Typen relationale Datenbanken, Graphdatenbanken und Dokumentdatenbanken im Detail anhand der Themen Datenbankmanagement, Datenbankmodellierung, Datenbanksprachen, Datenbanksicherheit und Systemarchitektur. Das Buch bietet außerdem einen Überblick auf postrelationale und nicht-relationale Datenbanksysteme. In den ersten fünf Kapiteln analysieren die Autoren detailliert die Verwaltung, Modellierung, Sprachen, Sicherheit und Architektur von relationalen Datenbanken, Graphdatenbanken und Dokumentendatenbanken. Außerdem wird ein Überblick über andere SQL- und NoSQL-basierte Datenbankansätze gegeben. Neben klassischen Konzepten wie dem Entitäts- und Beziehungsmodell und seiner Abbildung in SQL-Datenbankschemata, Abfragesprachen oder dem Transaktionsmanagement werden weitere Aspekte für NoSQL-Datenbanken wie nicht-relationale Datenbankschemas und Abfragesprachen (MQL, Cypher), das Map/Reduce-Verfahren, Verteilungsoptionen (Sharding, Replikation) oder das CAP-Theorem (Consistency, Availability, Partition Tolerance) erläutert. Die 9. Auflage bietet neu eine vertiefte Einführung in Dokumentdatenbanken mit einer Methode zur Modellierung von Dokumentstrukturen, einem Überblick auf dokumentenorientierte Datenbanksprache MongoDB Query Language MQL sowie Sicherheits- und Architekturaspekte. Die neue Auflage berücksichtigt neue Entwicklungen der Sprache Cypher. Das Thema Datenbanksicherheit wird neu als eigenes Kapitel eingeführt und im Detail bezüglich Datenschutz, Integrität und Transaktionen analysiert. Texte zum Datenmanagement, zur Datenbankprogrammierung und zum Data Warehouse wurden aktualisiert. Zudem erklärt die 9. Auflage die Konzepte JSON, JSON-Schema, BSON, indexfreie Nachbarschaft, Cloud-Datenbanken, Suchmaschinen und Zeitreihendatenbanken. Eine Webseite ergänzt den Inhalt des Buches durch Tutorien für Abfrage- und Manipulationssprachen (SQL, Cypher), Übungsumgebungen für Datenbanken (MySQL, Neo4j), zwei Fallstudien mit OpenOffice Base und Neo4j sowie als Unterrichtsmaterial Folienvorlagen mit allen Abbildungen Das Buch richtet sich sowohl an Studierende, die eine Einführung in das Gebiet der SQL- und NoSQL-Datenbanken möchten, als auch auch an Praktiker in Datenwissenschaften und Informatik, denen es hilft, Stärken und Schwächen relationaler und nicht-relationaler Ansätze sowie Entwicklungen für Big-Data-Anwendungen besser einschätzen zu können

Over the last decade Life Science has undergone an accelerated evolution, culminating in the -omics era characterized by the development of a multitude of high throughput methods that are becoming more routinely applied in biochemistry labs. In Functional Genomics: Methods and Protocols, Second Edition expert researchers in the field detail many of the methods which are now commonly used for studies in the life sciences focusing on the dynamic aspects of the transcriptome, proteome and metabolome, respectively.Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Functional Genomics: Methods and Protocols, Second Edition seeks to aid scientists in establishing or extending technologies and techniques in their laboratories.

This textbook offers a comprehensive introduction to relational (SQL) and non-relational (NoSQL) databases. The authors thoroughly review the current state of database tools and techniques and examine upcoming innovations. In the first five chapters, the authors analyze in detail the management, modeling, languages, security, and architecture of relational databases, graph databases, and document databases. Moreover, an overview of other SQL- and NoSQL-based database approaches is provided. In addition to classic concepts such as the entity and relationship model and its mapping in SQL database schemas, query languages or transaction management, other aspects for NoSQL databases such as non-relational data models, document and graph query languages (MQL, Cypher), the Map/Reduce procedure, distribution options (sharding, replication) or the CAP theorem (Consistency, Availability, Partition Tolerance) are explained. This 2nd English edition offers a new in-depth introduction to document databases with a method for modeling document structures, an overview of the document-oriented MongoDB query language MQL as well as security and architecture aspects. The topic of database security is newly introduced as a separate chapter and analyzed in detail with regard to data protection, integrity, and transactions. Texts on data management, database programming, and data warehousing and data lakes have been updated. In addition, the book now explains the concepts of JSON, JSON schema, BSON, index-free neighborhood, cloud databases, search engines and time series databases. The book includes more than 100 tables, examples and illustrations, and each chapter offers a list of resources for further reading. It conveys an in-depth comparison of relational and non-relational approaches and shows how to undertake development for big data applications. This way, it benefits students and practitioners working across the broad field of data science and applied information technology.

This book constitutes the thoroughly refereed post-proceedings of the 14th International Symposium on Graph Drawing, GD 2006, held in Karlsruhe, Germany. The 33 revised full papers and 5 revised short papers presented together with 2 invited talks, 1 system demo, 2 poster papers address all current aspects in graph drawing, ranging from foundational and methodological issues to applications for various classes of graphs in a variety of fields.

Graph drawing comprises all aspects of visualizing structural relations between objects. The range of topics dealt with extends from graph theory, graph algorithms, geometry, and topology to visual languages, visual perception, and information visualization, and to computer-human interaction and graphics design. This monograph gives a systematic overview of graph drawing and introduces the reader gently to the state of the art in the area. The presentation concentrates on algorithmic aspects, with an emphasis on interesting visualization problems with elegant solutions. Much attention is paid to a uniform style of writing and presentation, consistent terminology, and complementary coverage of the relevant issues throughout the 10 chapters.This tutorial is ideally suited as an introduction for newcomers to graph drawing. Ambitioned practitioners and researchers active in the area will find it a valuable source of reference and information.

This LNCS 13453 constitutes the thoroughly refereed proceedings of the 48th International Workshop on Graph-Theoretic Concepts in Computer Science, WG 2022.The 32 full papers presented in this volume were carefully reviewed and selected from a total of 96 submissions. The WG 2022 workshop aims to merge theory and practice by demonstrating how concepts from Graph Theory can be applied to various areas in Computer Science, or by extracting new graph theoretic problems from applications.

This book offers a comprehensive introduction to relational (SQL) and non-relational (NoSQL) databases. The authors thoroughly review the current state of database tools and techniques, and examine coming innovations. The book opens with a broad look at data management, including an overview of information systems and databases, and an explanation of contemporary database types: SQL and NoSQL databases, and their respective management systems The nature and uses of Big Data A high-level view of the organization of data management Data Modeling and Consistency Chapter-length treatment is afforded Data Modeling in both relational and graph databases, including enterprise-wide data architecture, and formulas for database design. Coverage of languages extends from an overview of operators, to SQL and and QBE (Query by Example), to integrity constraints and more. A full chapter probes the challenges of Ensuring Data Consistency, covering: Multi-User Operation Troubleshooting Consistency in Massive Distributed Data Comparison of the ACID and BASE consistency models, and more System Architecture also gets from its own chapter, which explores Processing of Homogeneous and Heterogeneous Data; Storage and Access Structures; Multi-dimensional Data Structures and Parallel Processing with MapReduce, among other topics. Post-Relational and NoSQL Databases The chapter on post-relational databases discusses the limits of SQL - and what lies beyond, including Multi-Dimensional Databases, Knowledge Bases and and Fuzzy Databases. A final chapter covers NoSQL Databases, along with Development of Non-Relational Technologies, Key-Value, Column-Family and Document Stores XML Databases and Graphic Databases, and more The book includes more than 100 tables, examples and illustrations, and each chapter offers a list of resources for further reading. SQL & NoSQL Databases conveys the strengths and weaknesses of relational and non-relational approaches, and shows how to undertake development for big data applications. The book benefits readers including students and practitioners working across the broad field of applied information technology. This textbook has been recommended and developed for university courses in Germany, Austria and Switzerland.

Plasmaphysik spielt heute in vielen Anwendungen eine wichtige Rolle, beispielsweise in dem weiten Feld der Astrophysik und zahlreichen modernen Technologien. Dieses Buch fuhrt in die Grundlagen des Themas ein und erschliesst den Zugang zu dem breiten Spektrum der Anwendungen.Einen Schwerpunkt bildet dabei die Fusionsforschung, die sich mit der Verschmelzung von Wasserstoffisotopen zur Energiegewinnung beschaftigt.Der Leser sollte mit der Elektrodynamik und den grundlegenden Kenntnissender statistischen Mechanik und der Quantenmechanik vertraut sein. "

Ein fundierter, interdisziplinarer Ansatz zur Optimierung der biologischen Schadstoffbehandlung:
Das neue ArtEv-Verfahren zum Abbau von Umweltgiften durch Mikroorganismen - erprobt, bewahrt und patentiert.
"

Take your DevOps and DevSecOps game to the next level by leveraging the power of the GitHub toolset in practice Key Features Release software faster and with confidence Increase your productivity by spending more time on software delivery and less on fixing bugs and administrative tasks Deliver high-quality software that is more stable, scalable, and secure Book DescriptionThis practical guide to DevOps uses GitHub as the DevOps platform and shows how you can leverage the power of GitHub for collaboration, lean management, and secure and fast software delivery. The chapters provide simple solutions to common problems, thereby helping teams that are already on their DevOps journey to further advance into DevOps and speed up their software delivery performance. From finding the right metrics to measure your success to learning from other teams' success stories without merely copying what they've done, this book has it all in one place. As you advance, you'll find out how you can leverage the power of GitHub to accelerate your value delivery - by making work visible with GitHub Projects, measuring the right metrics with GitHub Insights, using solid and proven engineering practices with GitHub Actions and Advanced Security, and moving to event-based and loosely coupled software architecture. By the end of this GitHub book, you'll have understood what factors influence software delivery performance and how you can measure your capabilities, thus realizing where you stand in your journey and how you can move forward. What you will learn Effectively measure software delivery performance Adopt DevOps and lean management techniques in your teams Plan, track, and visualize your work using GitHub Issues and Projects Use continuous delivery with GitHub Actions and Packages Scale quality through testing in production and chaos engineering "Shift left" security and secure your entire software supply chain Use DevSecOps practices with GitHub Advanced Security Secure your code with code scanning, secret scanning, and Dependabot Who this book is forThis book is for developers, solutions architects, DevOps engineers, and SREs, as well as for engineering or product managers who want to enhance their software delivery performance. Whether you're new to DevOps, already have experience with GitHub Enterprise, or come from a platform such as Azure DevOps, Team Foundation Server, GitLab, Bitbucket, Puppet, Chef, or Jenkins but struggle to achieve maximum performance, you'll find this book beneficial.

This is a reproduction of a book published before 1923. This book may have occasional imperfections such as missing or blurred pages, poor pictures, errant marks, etc. that were either part of the original artifact, or were introduced by the scanning process. We believe this work is culturally important, and despite the imperfections, have elected to bring it back into print as part of our continuing commitment to the preservation of printed works worldwide. We appreciate your understanding of the imperfections in the preservation process, and hope you enjoy this valuable book.