Focusing on "Saccharomyces cerevisiae," the second edition of Yeast Gene Analysis represents a major reworking of the original edition, with many completely new chapters and major revisions to all previous chapters. Originally published shortly after completion of the yeast genome sequence, the new edition covers many of the major genome-wide strategies that have been developed since then such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches. It represents a valuable resource for any research laboratory using budding yeast as their experimental system in which to identify new yeast gene functions. The chapters are written in a readable style with useful background information, technical tips and specific experimental protocols included as appropriate, enabling both the novice and the experienced yeast researcher to adopt new procedures with confidence.
New chapters on:
* strain construction
* genome-wide two-hybrid approaches
* use of microarrays for transcript analysis
* real-time analysis of chromosome behaviour and FRET
* synthetic gene array technology and protein arrays
* chemical genomics and yeast prions
* RNA gene analysis and mitochondrial gene function analysis
* phylogenetic footprinting
* discovering human gene function and predicting yeast gene function

The Derelict Light takes place in Prohibition-era Astoria, Oregon, a bleak and rain-soaked fishing town where violence and vice have found a home at the edge of the continent. After the Great War the city is awash in strife: civic leaders stoking economic ambitions, Finnish socialists gunning for a revolution, gamblers and boozers operating outside the law, and salmon fishermen just trying to survive the most dangerous river on the West Coast. Then comes the Ku Klux Klan in search of fresh recruits. When a fire destroys most of the city, and a body is found hanging from the docks, the city tears at its seams. Lines are drawn, and influence wields violence. Inspired by historical events, The Derelict Light explores a Pacific Northwest town in the grips of catastrophe, caught in a bitter struggle between progress, greed, and human frailty.

Reliable Object-Oriented Software presents the underlying principles associated with object-orientation and its practical application. More than just another text on methodology, this 1998 book focuses on the fundamental concepts related to the process of software development and architectural design in order to lay the basis necessary for the development of robust, maintainable, and evolvable software. The authors present both formal concepts and practical applications. It includes proven problem-solving techniques designed to develop readers' engineering judgement and to focus their attention on the principles of good software design and its successful application. In addition, an extensive bibliography has been compiled to guide the reader in further studies and to crystallize what is commonly accepted in the field and what is still controversial.