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This book provides a systematic introduction to the principles of
microscopic imaging through tissue-like turbid media in terms of
Monte-Carlo simulation. It describes various gating mechanisms
based on the physical differences between the un scattered and
scattered photons and method for microscopic image reconstruction,
using the concept of the effective point spread function. Imaging
an object embedded in a turbid medium is a challenging problem in
physics as well as in bio photonics. A turbid medium surrounding an
object under inspection causes multiple scattering, which degrades
the contrast, resolution and signal-to-noise ratio. Biological
tissues are typically turbid media. Microscopic imaging through a
tissue-like turbid medium can provide higher resolution than
transillumination imaging in which no objective is used. This book
serves as a valuable reference for engineers and scientists working
on microscopy of tissue turbid media.
Optical microscopy and associated technologies have advanced
rapidly along with laser technology. These techniques have
stimulated further development of the optical imaging theory,
including 3-dimensional microscopy imaging theory, the theory of
imaging with ultrashort pulsed beam illumination and the aberration
theory for high numerical-aperture objectives. This book introduces
these new theories in modern optical microscopy, providing
comparisons with classical imaging as appropriate.
Optical microscopy and associated technologies advanced quickly after the introduction of the laser. The techniques have stimulated further development of optical imaging theory, including 3-dimensional microscopy imaging theory in spatial and frequency domains, the theory of imaging with ultrashort-pulse beams and aberration theory for high-numerical-aperture objectives. This book introduces these new theories in terms of modern optical microscopy. It consists of seven chapters including an introduction. The chapters are organized to minimize cross-referencing. Comparisons with classical imaging theory are made when the new imaging theory is introduced. The book is intended for senior undergraduate students in courses on optoelectronics, optical engineering, photonics, biophotonics and applied physics, after they have completed modern optics or a similar subject. It is also a reference for other scientists interested in the field.
This book discusses the various principles in confocal scanning
microscopy which has become a useful tool in many practical fields
including biological studies and industrial inspection. The
methodology presented in this book is unique and is based on the
concept of the three-dimensional transfer functions which have been
developed by the author and his colleagues over the last five
years. With the 3-D transfer functions, resolving power in 3-D
confocal imaging can be defined in a unified way, different optical
arrangements can be compared with an insight into their
inter-relationship, and images of thick objects can be modeled in
terms of the Fourier transform which makes the analysis easy. The
aim of this book is to provide a systematic introduction to the
concept of the 3-D transfer functions in various confocal
microscopes, to describe the methods for the derivation of
different 3-D transfer functions, and to explain the principles of
3-D confocal imaging in terms of these functions.
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Gloria
Sam Smith
CD
R407
Discovery Miles 4 070
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