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The book aimed to reveal degradation of Cucurbitacin E glucoside
obtained from Citrullus colocynthis (L.) (Hanzal) into Cucurbitacin
E and glucose by mean of microbial biotransformation using
Curvularia lunata NRRL 2178 as a source of -glucosidase. All the
instrumental data obtained by highly advanced techniques, confirmed
the microbial conversion of cucurbitacin E glucoside into
cucurbitacin E and glucose indicating effect of enzyme in the
hydrolysis process by splitting of glucose from the anomeric carbon
atom. The effect of the cucurbitacin E glucoside and cucurbitacin E
on the proliferation of Hep-G2 cells was studied. The treatment of
Hep-G2 cells with cucurbitacin E led to a high inhibition of the
cell proliferation, which revealed a moderate anti-tumor activity
of the cucurbitacin E against hepatic carcinoma, while cucurbitacin
E glucoside had no cytotoxic effect on Hep-G2 cells. The study
showed the effect of the compounds on the proliferation of HCT-116
cells; the two compounds were not cytotoxic as indicated by their
IC50 values. On the other hand, the treatment of T-lymphocyte cells
with cucurbitacin E resulted in increase in the cell
proliferation."
The book is intended as that introduction to the ICP-OES technique.
It was written not only for those persons who have some familiarity
with other analytical techniques such as atomic absorption
spectrometry but also for novices in the field of analytical
chemistry. The book begins with some simple, yet fundamental,
concepts regarding atomic spectroscopy and the analytical
techniques based on this field of study. As one progresses through
the book, more detail regarding the ICP-OES technique is presented
including information about ICP-OES performance, instrumentation
and methodology. We have also included some information about
instrument maintenance and performance verification. While this
kind of practical information can be vital to obtaining good
analytical results, it is sometimes difficult to find. We hope that
this book will provide useful information to those persons who are
about to get involved with ICP-OES as well as present ICP users and
those with simply a curiosity about the technique.
The book shows principles and applications of the most distributed
technique used for water analysis. The central laboratory of
Greater Cairo Drinking Water Company (GCWC) uses this important
technique and applies the analytical methods recorded in the
standard methods for water and wastewater examination. This
laboratory represents nucleus and origin of many reference
laboratories of water analysis in Egypt. Wael Mahmoud and his team
of the researchers modified the scientific cooperation between the
central laboratory of GCWC and the national research centre. Wael
and his team used the chromatographic techniques in the central
laboratory to make their ideas and researches in field of the water
examination. They added new concepts in the technical uses of the
chromatographic techniques and without scientific experience of
this team, the central laboratory still do the routine and
traditional analytical work without any improvement. The book
confirms the concept which explains that it is impossible for the
traditional technical work to progress without science.
The current study added complete idea about different reasons
related to increasing the disinfection by-products (DBPs). It also
evaluated ratio of the individual by-products to the total organic
halogen. The study suggested new methods to minimize some of these
by-products. It also added new idea about effect of the different
chlorine doses on the electrophoretic protein pattern. The most
important goal of the study is suggestion of new disinfecting agent
without DBPs to replace chlorine in the difficult conditions. The
study covered effect of the new disinfecting agent on the
electrophoretic DNA pattern and the different body functions at
level of the experimental animals and compared it with effect of
chlorine. Finally, the authors' group added new experimental idea
to change the traditional concepts of water treatment and water
analysis to be applicable in all water treatment plants.
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