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In this study hyper-production of citric acid Aspergillus niger was
carried out using agro-industrial residues as carrier substrates in
solid state fermentation (SSF) of sucrose molasses medium. It was
concluded from this study that banana stalk was a good carrier
substrate among the six substrates (Corncobs, corn stover, wheat
straw, wheat bran and sugarcane bagasse) used for citric acid
production in SSF of molasses medium and SSF gave higher citric
acid production as compared to liquid state fermentation (LSF).
Mutants of Aspergillus niger were produced through UV irradiations,
Ethyle methane sulfonate (EMS) and Ethidium bromide (EB) for
different time periods and Among the different mutants selected
using selective marker, EB treated mutant Aspergillus niger EB-3
(treated for 90 minutes) was the best mutant for enhanced
production of citric acid. Moreover, citric acid production by the
mutant could be substantially enhanced to 112 mg/mL by careful
optimization of the SSF parameters and by the addition of different
metabolic inhibitors and metal ion complexing compounds.
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