|
Showing 1 - 12 of
12 matches in All Departments
In vitro propagation of vitis is applied to increase plant material
for cultivation. Cultures were maintained in vitro on MS medium
supplemented with various growth regulators such as 2,4-D, BAP,
NAA, TDZ and Kin. Comparatively all the hormones alone or in
combinations were tried on grape varieties to examine their effects
on initiation, as well as on multiplication. The 100% explant
sterilization was achieved when free leaf explants of V1 and V2
exposed to 5% sodium hypochlorite for the duration of 5 minute. For
V3 the same combination of treatment was found to be effective with
90 % survival of explants. It was observed during the present study
that the nodal explants of all the three grape varieties gave 100%
contamination free survival when they were treated with 5% sodium
hypochlorite for 10 minutes. Best Shoot induction was observed in
MS 1 medium i.e BAP 1mg/l. While maximum rooting was observed in
NAA 1mg/l. The highest callus induction by leaf explants was 95%
with V2 on 2.4-D 3mg/l followed by nodal segments on BA 1mg/l + NAA
0.5mg/l. Callogenesis from nodal explants regenerated in roots. By
physical mutagenesis it was noted that doses up to 5 Gy helped to
increase callus
|
You may like...
Peacekeeping
Various
Hardcover
R7,602
Discovery Miles 76 020
|