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In vitro propagation of vitis is applied to increase plant material
for cultivation. Cultures were maintained in vitro on MS medium
supplemented with various growth regulators such as 2,4-D, BAP,
NAA, TDZ and Kin. Comparatively all the hormones alone or in
combinations were tried on grape varieties to examine their effects
on initiation, as well as on multiplication. The 100% explant
sterilization was achieved when free leaf explants of V1 and V2
exposed to 5% sodium hypochlorite for the duration of 5 minute. For
V3 the same combination of treatment was found to be effective with
90 % survival of explants. It was observed during the present study
that the nodal explants of all the three grape varieties gave 100%
contamination free survival when they were treated with 5% sodium
hypochlorite for 10 minutes. Best Shoot induction was observed in
MS 1 medium i.e BAP 1mg/l. While maximum rooting was observed in
NAA 1mg/l. The highest callus induction by leaf explants was 95%
with V2 on 2.4-D 3mg/l followed by nodal segments on BA 1mg/l + NAA
0.5mg/l. Callogenesis from nodal explants regenerated in roots. By
physical mutagenesis it was noted that doses up to 5 Gy helped to
increase callus
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