This Open Access textbook provides students and researchers in the life sciences with essential practical information on how to quantitatively analyze data images. It refrains from focusing on theory, and instead uses practical examples and step-by step protocols to familiarize readers with the most commonly used image processing and analysis platforms such as ImageJ, MatLab and Python. Besides gaining knowhow on algorithm usage, readers will learn how to create an analysis pipeline by scripting language; these skills are important in order to document reproducible image analysis workflows. The textbook is chiefly intended for advanced undergraduates in the life sciences and biomedicine without a theoretical background in data analysis, as well as for postdocs, staff scientists and faculty members who need to perform regular quantitative analyses of microscopy images.

This open access textbook aims at providing detailed explanations on how to design and construct image analysis workflows to successfully conduct bioimage analysis. Addressing the main challenges in image data analysis, where acquisition by powerful imaging devices results in very large amounts of collected image data, the book discusses techniques relying on batch and GPU programming, as well as on powerful deep learning-based algorithms. In addition, downstream data processing techniques are introduced, such as Python libraries for data organization, plotting, and visualizations. Finally, by studying the way individual unique ideas are implemented in the workflows, readers are carefully guided through how the parameters driving biological systems are revealed by analyzing image data. These studies include segmentation of plant tissue epidermis, analysis of the spatial pattern of the eye development in fruit flies, and the analysis of collective cell migration dynamics. The presented content extends the Bioimage Data Analysis Workflows textbook (Miura, Sladoje, 2020), published in this same series, with new contributions and advanced material, while preserving the well-appreciated pedagogical approach adopted and promoted during the training schools for bioimage analysis organized within NEUBIAS - the Network of European Bioimage Analysts. This textbook is intended for advanced students in various fields of the life sciences and biomedicine, as well as staff scientists and faculty members who conduct regular quantitative analyses of microscopy images.

This book constitutes the proceedings of the First IAPR International Conference on Discrete Geometry and Mathematical Morphology, DGMM 2021, which was held during May 24-27, 2021, in Uppsala, Sweden.The conference was created by joining the International Conference on Discrete Geometry for computer Imagery, DGCI, with the International Symposium on Mathematical Morphology, ISMM. The 36 papers included in this volume were carefully reviewed and selected from 59 submissions. They were organized in topical sections as follows: applications in image processing, computer vision, and pattern recognition; discrete and combinatorial topology; discrete geometry - models, transforms, visualization; discrete tomography and inverse problems; hierarchical and graph-based models, analysis and segmentation; learning-based approaches to mathematical morphology; multivariate and PDE-based mathematical morphology, morphological filtering. The book also contains 3 invited keynote papers.

This book constitutes the refereed proceedings of the 20th International Workshop on Combinatorial Image Analysis, IWCIA 2020, held in Novi Sad, Serbia, in July 2020. The 20 full papers presented were carefully reviewed and selected from 23 submissions. The papers are grouped into two sections. The first one includes twelve papers devoted to theoretical foundations of combinatorial image analysis, including digital geometry and topology, array grammars, picture languages, digital tomography, and other technical tools for image analysis. The second part includes eight papers presenting application-driven research on topics such as image repairing, annotation of images, image reconstruction, forgery detection, and dealing with noise in images.