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This second edition volume expands on the previous edition with a
discussion of new research and discoveries in the Rab field.
Chapters in this book cover topics such as new information on Rab
regulation and localization; interaction; function; and diseases.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
comprehensive, Rab GTPases: Methods and Protocols, Second Edition
is a valuable resource for scientists working in the fields of Rab
and other small GTPases, and beyond.
The SecretoryPathway The transport ofproteins and lipids from their
site ofsynthesis at the endoplasmic reticu- lum (ER) to the
cellsurface ismediated by the secretory pathway and isan essential
process in eukaryotic organisms. A great variety ofmolecules are
extruded from cellsby the action ofthe secretory pathway, including
extracellular matrix components that provide the foundation for
constructing tissuesand organs. Moreover, this pathway playsa major
role in the biogenesisof the plasma membraneand itsexpansion before
celldivision. Therefore, withoutsecretion there would be no
cells,tissuesor organs, and so it issafeto saythat we oweourvery
existence to the secretory pathway. To understand the process
ofsecretion we must learn about the organelles that compose the
secretory pathway; the ER and Golgi apparatus, and the transport
vesicles these or- ganelles produce. The membrane ofthese
organelles is primarily synthesized and assembled at the ER but
with contributions from mitochondria (phosphatidylethanolamine) and
the Golgi apparatus (sphingolipids). Newly synthesized proteins
destined for secretion gain en- try into the secretory pathway by
translocation across the ER membrane. This translocation apparatus
also integrates proteins into the membrane and establishes their
topology with respect to the lipid bilayer (seeChapter 7). Many
secretory proteins are covalently modified with oligosaccharides to
produce glycoproteins, a biosynthetic process initiated in the ER
and continued in the Golgi apparatus. Once proteins are properly
folded and modified in the ER, they are allowed to leave and are
ushered into COPlI-coated carrier vesiclesforming at specific exit
sites (see Chapters 1 and 8).
This second edition volume expands on the previous edition with a
discussion of new research and discoveries in the Rab field.
Chapters in this book cover topics such as new information on Rab
regulation and localization; interaction; function; and diseases.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
comprehensive, Rab GTPases: Methods and Protocols, Second Edition
is a valuable resource for scientists working in the fields of Rab
and other small GTPases, and beyond.
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