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This book is a comprehensive guide for all tissue bank operators to
screen, procure and process amniotic membrane for clinical
application.The amnion comes close to being the ideal biological
membrane or dressing - readily available, inexpensive to procure
and process. Its basic science is discussed in detail - anatomy,
biological and biomechanical properties.It can be procured from the
placenta in normal vaginal deliveries and from Caesarean Sections.
Processing is by freeze-drying or by air-drying process with
sterilisation using gamma irradiation.The product has low
antigenicity, has anti-microbial properties with ability to enhance
epithelisation with marked relief of pain. It is useful as a
dressing for wounds - flap wounds, burn wounds, injury wounds,
diabetic ulcers, leprous ulcers and post-surgery wounds and
post-radiation wounds. It is also used as a biological scaffold for
cells in tissue engineering. Its ophthalmic applications include
treatment of corneal ulcers and conjunctival tumours. Oral uses
include gingiva depigmentation and periodontal regeneration.
This book is written as a comprehensive guide for all tissue bank
operators to procure and process bone and soft tissue allografts of
highest quality standards for safe tissue transplantation practice
in patients who require musculoskeletal tissue allograft
transplantation. This comprehensive guide includes donor selection
criteria, aseptic procurement techniques, laboratory testing and
processing of grafts by deep freezing or freeze drying. It also
includes sterilization of tissue grafts using gamma irradiation.
Quality controls of tissue grafts are discussed in depth. The
clinical transplantation of bone and soft tissue allografts is also
discussed, with special consideration given to potential
complications. Principles of sterile technique in the operating
theater are described. The book also incorporates the basic
sciences of tissue banking including anatomy, biomechanics,
microbiology and immunology. It also covers radiation science so
that the reader can better understand radiation sterilization of
tissue grafts. Included in the book is a guide for public awareness
programmes, radiation code of practice and general standards for
tissue banking as recommended by the International Atomic Energy
Agency.
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