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This fourth edition provides new and updated protocols on plant
cell, tissue, and organ cultures. Chapters are divided into five
parts that cover topics from general methodologies, statistical
analysis and contamination control, highly specialized techniques,
and laborious process of measuring the epigenetics changes in
tissue cultures. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant Cell Culture Protocols,
Fourth Edition aims to serve both professionals and novices with
its guide to the most common and applicable techniques and methods
for plant tissue and cell culture.
This book discusses basic and applied aspects of somatic
embryogenesis, one of the most powerful tools in plant
biotechnology. It is divided into three parts; Part I includes
topics such as the history of this research field, how
differentiated plant cells can (re)acquire totipotency, molecular
features, as well as the epigenetics and proteomics of somatic
embryogenesis. Part II covers the somatic embryogenesis of
different crops, such as Agave spp. maize, Cocos nucifera, Bixa
orellana, Capsicum spp., Coffea spp., Musa spp., Pinus spp., and
Arabidopsis thaliana. Various applications, like scale-up
propagation and genetic engineering are discussed in detail in Part
III. The book will appeal to plant scientists, plant breeders and
experts working in industry.
Cell culture methodologies have become standard procedures in most
plant laboratories. Currently, facilities for in vitro cell
cultures are found in practically every plant biology laboratory,
serving different purposes since tissue culture has turned into a
basic asset for modern biotechnology, from the fundamental
biochemical aspects to the massive propagation of selected
individuals. "Plant Cell Culture Protocols, Third Edition is
divided into five convenient sections that cover topics from
general methodologies, such as culture induction, growth and
viability evaluation, statistical analysis and contamination
control, to highly specialized techniques, such as clonal
propagation, haploid production, somatic embryogenesis, organelle
transformation. The volume concludes with a section on the
laborious process of measuring the epigenetics changes in tissue
cultures."Written in the successful Methods in Molecular Biology
(TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls. Authoritative and
easily accessible, Plant Cell Culture Protocols, Third Edition
seeks to serve both professionals and novices with its guide to the
most common and applicable techniques and methods for plant tissue
and cell culture.
This book discusses basic and applied aspects of somatic
embryogenesis, one of the most powerful tools in plant
biotechnology. It is divided into three parts; Part I includes
topics such as the history of this research field, how
differentiated plant cells can (re)acquire totipotency, molecular
features, as well as the epigenetics and proteomics of somatic
embryogenesis. Part II covers the somatic embryogenesis of
different crops, such as Agave spp. maize, Cocos nucifera, Bixa
orellana, Capsicum spp., Coffea spp., Musa spp., Pinus spp., and
Arabidopsis thaliana. Various applications, like scale-up
propagation and genetic engineering are discussed in detail in Part
III. The book will appeal to plant scientists, plant breeders and
experts working in industry.
Cell culture methodologies have become standard procedures in most
plant laboratories. Currently, facilities for in vitro cell
cultures are found in practically every plant biology laboratory,
serving different purposes since tissue culture has turned into a
basic asset for modern biotechnology, from the fundamental
biochemical aspects to the massive propagation of selected
individuals. "Plant Cell Culture Protocols, Third Edition is
divided into five convenient sections that cover topics from
general methodologies, such as culture induction, growth and
viability evaluation, statistical analysis and contamination
control, to highly specialized techniques, such as clonal
propagation, haploid production, somatic embryogenesis, organelle
transformation. The volume concludes with a section on the
laborious process of measuring the epigenetics changes in tissue
cultures."Written in the successful Methods in Molecular Biology
(TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls. Authoritative and
easily accessible, Plant Cell Culture Protocols, Third Edition
seeks to serve both professionals and novices with its guide to the
most common and applicable techniques and methods for plant tissue
and cell culture.
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